Applications of signal transduction engineering and genetic engineering in production of ganoderic acid in submerged culture of Ganoderma lucidum
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摘要: 灵芝酸是灵芝中的主要活性成分之一,现代药理和临床研究表明,灵芝酸具有毒杀肿瘤细胞、抑制癌细胞转移、抗HIV-1和HIV-1蛋白酶等功能。目前悬浮培养灵芝细胞法是大规模生产灵芝酸的重要策略,具有很好的应用潜力和前景。近些年,有大量的文献报道通过不同方式提高灵芝细胞中灵芝酸的含量,并取得了一定进展。文章主要从信号转导调控、诱导子策略和基因工程等方面综述了近年来悬浮培养生产灵芝酸的研究进展,并对以后通过调控代谢通路和合成生物学实现灵芝酸的高效生产进行了展望。Abstract: Ganoderic acids( GAs) are important bioactive constituents produced by Ganoderma spp.Modern pharmacological and clinical studies have shown that GAs had extraordinarily pharmacological functions,such as anti- viral,anti- tumor,immuno- modulating effect,etc. Submerged fermentation of G. lucidum is a promising technology for efficient production of GAs. Recent publication about fermentation production of GAs in the last decade,especially the progresses toward signal transduction strategies,induction strategies and genetic engineering were summarized in this paper.Moreover,metabolic engineering and synthetic biology approaches for efficient production of GAs were also proposed in this paper.
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Keywords:
- Submerged culture /
- biosynthesis /
- signal transduction /
- induction strategies /
- genetic engineering
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