Antioxidant activity of ethanol extracts from Jatropha curcus L.seed shell
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摘要: 本实验以麻疯树籽壳乙醇提取物中黄酮质量浓度为内标,以维生素C（VC）和2,6-二叔丁基-4-甲基苯酚（BHT）为对照,通过超氧阴离子（O-2·）、1,1-二苯基-2-苦基肼自由基（DPPH·）和亚硝酸盐（NO-2）的清除能力,以及还原力和总抗氧化能力的测定,评价了麻疯树籽壳乙醇提取物的抗氧化活性。实验结果表明,麻疯树籽壳乙醇提取物的O-2·清除率的半数抑制浓度（IC50）为0.502mg/m L,小于VC的0.654mg/m L;其DPPH·与NO-2清除率的IC50分别为0.185、0.494mg/m L,均小于BHT;麻疯树籽壳乙醇提取物中黄酮质量浓度在0.71.0mg/m L时,其还原力与同质量浓度VC相当,大于BHT;在测定范围时,其总抗氧化能力随质量浓度增加而增强,且大于同质量浓度的BHT,而小于VC。由此说明,麻疯树籽壳乙醇提取物具有较强的抗氧化活性。Abstract: Antioxidant activity of ethanol extracts from Jatropha curcus L. seed shell was determined by evaluating the scavenging activity of superoxide anion radical ( O-2·) , 2, 2- diphenyl-2- picrylhydrazyl radical ( DPPH·) and nitrite ( NO-2) , reducing power and total antioxidant capacity, taking the flavonoids concentration of its ethanol extracts for internal standard and comparing to vitamin C ( VC) and Butylated hydroxytoluene ( BHT) .The results showed that the half inhibitory concentration ( IC50) values of ethanol extracts from Jatropha curcus L.seed shell on O-2·was 0.502 mg /m L, lower than that of VC ( 0.654 mg /m L) .The IC50 values of ethanol extracts on DPPH·and NO-2were 0.185 and 0.494 mg / m L, respectively; both of them were lower than BHT's.When the flavonoids concentration of ethanol extracts in the range of 0.7 to 1.0 mg / m L, its reducing power was equal to that of VC, but stronger than that of BHT. In addition, the total antioxidant power increased with increasing of flavonoids concentration from0.1mg / m L to 1.0mg / m L, which was higher than that of BHT, lower than that of VCat the same concentration though.All of above indicated that ethanol extracts from Jatropha curcus L. seed shell had stronger antioxidant activity.
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Keywords:
- Jatropha curcus L. /
- seed shell /
- ethanol extracts /
- antioxidant activity
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[1] 全治国, 赵静峰, 羊晓东, 等.小桐子的化学成分及药理作用研究进展[J].云南化工, 2009, 36 (2) :39-43. [2] 李玲, 李晓帆, 吴慧星, 等.麻疯树种子中抗氧化活性成分的研究[J].中草药, 2010, 41 (12) :1932-1936. [3] 马博, 兰翠玲, 李力, 等.麻疯树籽粕饲用品质改良及其深加工技术研究进展[J].中国油脂, 2011, 36 (5) :26-30. [4] 刘细祥, 马博, 张婷婷, 等.响应面法优化麻疯树籽壳总黄酮工艺的研究[J].食品工业科技, 2014, 35 (6) :284-287, 291. [5] 张婷婷, 马博, 李荣峰, 等.桂西野生蕨菜黄酮提取工艺的研究[J].北方园艺, 2013, 16:163-165. [6] 宣丽, 刘长江.不同提取方法对软枣猕猴桃多糖单糖组成及抗氧化活性的影响[J].天然产物研究与开发, 2013, 25:1260-1265. [7] 郭峰, 岳永德, 汤峰, 等.用清除有机自由基DPPH法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析, 2008, 28 (7) :1578-1582. [8] 李利华.马铃薯块茎粗多分体外抗氧化活性[J].食品与发酵工业, 2013, 37 (9) :176-179. [9] Benzie IF, Strain JJ.The ferric reducing ability of plasma (FRAP) as a measure of“antioxidant power”:the FRAP assay[J].Analytical Biochemistry, 1996, 239 (1) :70-76.
[10] Choe JH, Jang A, Choi JH, et al.Antioxidant Activities of Lotus Leaves (Nelumbo nucifera) and Barley Leaves (Hordeum vulgare) Extracts[J].Food Science Biotechnology, 2010, 19 (3) :831-836.
[11] Jagtap UB, Panaskar SN, Bapat VA.Evaluation of Antioxidant Capacity and Phenol Content in Jackfruit (Artocarpus heterophyllus Lam.) Fruit Pulp[J].Plant Foods for Human Nutrition, 2010, 65:99-104.
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