Detection of Salmonella by double antibody sandwich ELISA
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摘要: 沙门氏菌是自然界中普遍存在的一种食源性致病菌,在中国的食物中毒中沙门氏菌引起的病例占第一位。本研究以福尔马林灭活的鼠伤寒沙门氏菌免疫雌性日本大耳兔,制备抗沙门氏菌多克隆抗体。以抗沙门氏菌多克隆抗体作为捕获抗体,以抗沙门氏菌单克隆抗体C1359作为检测抗体,建立快速检测沙门氏菌双抗夹心ELISA方法。结果表明:该方法可以检测A、B、C、D、E等五种典型的沙门氏菌,对沙门氏菌纯培养液的最低检测量为1×104CFU/mL,与其他杂菌不存在交叉反应,具有较好的灵敏性和特异性。
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关键词:
- 沙门氏菌 /
- 多克隆抗体 /
- 双抗夹心酶联免疫吸附法
Abstract: Salmonella is a kind of common foodborne pathogens in nature which causes the greatest number of food poisoning cases in China. Female Japanese white rabbits were immunized with formalin inactivated Salmonella typhimurium as antigens to prepare polyclonal antibody. The anti-Salmonella polyclonal antibody was used as capture antibody and C1359 monoclonal antibody was used as detection antibody to develop rapid double antibody sandwich ELISA. It showed that A, B, C, D, and E typical strains of Salmonella could be detected and the limit of detection was 1 ×104CFU/mL in medium, and there was no cross reaction with miscellaneous bacteria. It could be concluded that this method was with high sensitivity and specificity.-
Keywords:
- Salmonella /
- polyclonal antibody /
- double antibody sandwich ELISA
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