Water extracts from Grifola frondosa and antimicrobial function
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摘要: 为研究水溶液提取灰树花营养成分,采用水溶液对灰树花多糖进行了单因素及正交实验提取,用苯酚-硫酸法对多糖进行了测定。并用滤纸片法研究了灰树花水提物的抑菌能力。正交实验结果表明,影响灰树花多糖提取率的因素大小顺序依次是:温度、液料比、提取时间。灰树花多糖的提取最优条件为:温度80℃、液料比60∶1（mL∶g）、提取时间2.0h。灰树花水提物对枯草芽孢杆菌、大肠杆菌和金黄色葡萄球菌都有不同程度的抑制作用,抑菌圈直径分别为8.74、10.23、12.42mm。Abstract: The purpose of this research was to extract polysaccharide from Grifola frondosa, single factor and orthogonal tests were carried out. The method of phenol-sulfuric acid was used to determine the content of polysaccharide. Antibacterial performance test about water extracts was studied, too. Orthogonal tests showed that optimum conditions of polysaccharide extraction were as follows : extraction temperature 80 ℃, liquid ratio 60∶1 (mL∶g) and extraction time 2.0h. Water extracts from G. frondosa had antibacterial activity to Bacillus subtilis, Escherichia coli and Staphylococcus aureus, and antibacterial circle were 8. 74, 10. 23, 12. 42mm respectively.
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Keywords:
- Grifola frondosa /
- water extracts /
- orthogonal test /
- polysaccharide /
- antimicrobial function
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