Isolation and structural identification of alkaloids of papaya leaves
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摘要: 干燥的番木瓜叶用含1%HCl的90%乙醇溶液于常温下提取5次,浓缩、过滤、石油醚脱脂,NH3·H2O调pH至8.0~9.0,三氯甲烷萃取,得生物碱粗提物。采用硅胶柱柱层析对粗体物进一步进行分离纯化,得到番木瓜碱,并通过IR、MS和NRM对其进行了结构鉴定。Abstract: Alkaloids were extracted five times from the dry papaya leaves by the solution of 90% ethanol contains1% HCl. And then, the extract was concentrated, filtered, and degreased by petroleum ether. The acidity was adjusted to pH8.09.0 using concentrate NH3·H2O solution, and then extracted with excess chloroform. After purification of Silica gel column chromatography, alkaloids structural identification was performed with spectral methods (IR、MS、NMR) . The carpaine was isolated and identified.
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Keywords:
- papaya leaves /
- alkaloids /
- carpaine /
- structure identification
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