Preparation of Composite Preservation Paper containing Antagonistic Fungi and Its Application on Apple Preservation

LIU Yixuan; YUE Hong; SI Xinyu; ZHOU Menghan; CHEN Cunkun; GUO Honglian

doi:10.13386/j.issn1002-0306.2024050058

Turn off MathJax

Article Contents

Abstract

References

LIU Yixuan, YUE Hong, SI Xinyu, et al. Preparation of Composite Preservation Paper containing Antagonistic Fungi and Its Application on Apple Preservation[J]. Science and Technology of Food Industry, 2025, 46(6): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050058.

Citation:

PDF (16774 KB)

Preparation of Composite Preservation Paper containing Antagonistic Fungi and Its Application on Apple Preservation

1.
College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China
2.
Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, National Engineering and Technology Research Center for Preservation of Agricultural Products (Tianjin), Tianjin 300384, China

More Information

Received Date: May 08, 2024
Available Online: January 07, 2025

Graphical Abstract

Abstract

Abstract

In order to study the properties of composite preservation paper containing antagonistic fungi and the effect of the composite preservation paper on the physiological quality and preservation quality of Gala apples at room temperature, and at the same time to solve the limitations of the antagonistic fungi in the application of difficult to germinate, subject to the influence of the environment, etc., this study used Pichia membranaefaciens as the antagonistic fungi, added to the mixture of 1.16% gelatin and 2% trehalose solution, and combined with a paper-based material to prepare a composite preservation paper containing antagonistic fungi. The structure was characterized, and the physical properties and thermal stability performance of the composite preservation paper were measured. Effects of composite preservation on the natural disease incidence of Gala apples, spot diameter, weight loss, hardness, browning degree, reducing sugar, titratable acid, vitamin C and soluble solids content were analyzed to comprehensively evaluate the preservation effect of the composite preservation paper on Gala apples. Results showed that the composite preservation liquid containing antagonistic fungi caused the surface of Alternaria spp. spores to crumple and deform, and the morphology was destroyed. The composite preservation liquid containing antagonistic fungi at a concentration of 10⁸ CFU/mL was tightly combined with the paper-based material. The water absorption rate of the composite preservation paper was 768.94%±38.05%, which was at a high level. The thickness of the composite preservation paper was 0.232±0.008 mm, the tensile strength was 13.88±0.59 N/mm², and the percentage of breaking elongation was 6.33%±0.58%, indicating that it had good physical properties. There was a major mass loss in the range of 320~450 ℃, and the residual rate was 5.31% at 800 ℃, with good thermal stability. In vivo application showed that the composite preservation paper could effectively delay the expansion of spots in inoculated fruits, reduce the weight loss rate and browning degree of fruits and maintain the hardness of fruits, delay the reduction of vitamin C, reducing sugar and soluble solids content of fruits, maintain the good organoleptic and physiological qualities of fruits, and prolong the storage time. The composite preservation paper has good physical properties and thermal stability, and maintains the storage quality of Gala apples after harvesting, which not only provides new ideas for antagonistic yeast inhibition and preservation, but also provides a reference for the development of new biological preservation paper and preservation application.

FullText(HTML)

References (40)

References

[1]	HUANG X Q, REN J, LI P H, et al. The potential of microbial endophytes to enhance the resistance to postharvest diseases of fruit and vegetables[J]. Journal of the Science of Food and Agriculture,2021,101(5):1744−1757. doi: 10.1002/jsfa.10829
[2]	ZHANG X K, LI B Q, ZHANG Z Q, et al. Antagonistic yeasts:A promising alternative to chemical fungicides for controlling postharvest decay of fruit[J]. Journal of Fungi,2020,6(3):158. doi: 10.3390/jof6030158
[3]	LI Y T, XIA X N, ZHAO Q M, et al. The biocontrol of potato dry rot by microorganisms and bioactive substances:A review[J]. Physiological and Molecular Plant Pathology,2022,122:101919. doi: 10.1016/j.pmpp.2022.101919
[4]	李超兰. 抗坏血酸对拮抗菌卡利比克毕赤酵母生防效力及氧化胁迫耐受性影响的研究[D]. 镇江:江苏大学, 2014. [LI C L. The effect of V_C on the biocontrol activity and the oxidative tolerance of the antagonist Pichia caribbica[D]. Zhenjiang:Jiangsu University, 2014.] LI C L. The effect of V_C on the biocontrol activity and the oxidative tolerance of the antagonist Pichia caribbica[D]. Zhenjiang: Jiangsu University, 2014.
[5]	张国超. 甜菜碱对卡利比克毕赤酵母生防效力及氧化胁迫耐受性影响的研究[D]. 镇江:江苏大学, 2015. [ZHANG G C. The effect of GB on the biocontrol activity and the oxidative tolerance of the antagonist Pichia caribbica[D]. Zhenjiang:Jiangsu University, 2015.] ZHANG G C. The effect of GB on the biocontrol activity and the oxidative tolerance of the antagonist Pichia caribbica[D]. Zhenjiang: Jiangsu University, 2015.
[6]	ZONG Y Y, LIU J, LI B Q, et al. Effects of yeast antagonists in combination with hot water treatmenton postharvest diseases of tomato fruit[J]. Biological Control,2010,54:316−321. doi: 10.1016/j.biocontrol.2010.06.003
[7]	LUO Y, ZENG K F, MING J. Control of blue and green mold decay of citrus firuit by Pichia membranefaciens and induction of defense responses[J]. Scientia Horticulturae,2012,135:120−127. doi: 10.1016/j.scienta.2011.11.031
[8]	SUI Y, LIU J, WISNIEWSKI M, et al. Pretreatment of the yeast antagonist, Candida olephila, withglycine betaine increases oxidative stress tolerance in the microenvironment of apple wounds[J]. International Journal of Food Microbiology,2012,157:45−51. doi: 10.1016/j.ijfoodmicro.2012.04.010
[9]	FENG M F, LÜ Y, LI T T, et al. Postharvest treatments with three yeast strains and their combinations to control Botrytis cinerea of snap beans[J]. Foods,2021,10(11):2736. doi: 10.3390/foods10112736
[10]	GUO H L, CUI Y, ZHU E L, et al. Effect of antagonistic yeast Pichia membranaefaciens on black spot decay of postharvest broccoli[J]. European Journal of Plant Pathology,2015,143:373−383. doi: 10.1007/s10658-015-0689-5
[11]	陈海艺, 徐悦, 刘艺璇, 等. 膜醭毕赤酵母蛋白复合膜对桃采后果腐病的抑制效果及机制[J]. 食品与发酵工业,2024,50(5):274−279. [CHEN H Y, XU Y, LIU Y X, et al. Inhibitory effect and mechanism of Pichia membranaefaciens composite protein film on postharvest rot of peaches[J]. Food and Fermentation Industries,2024,50(5):274−279.] CHEN H Y, XU Y, LIU Y X, et al. Inhibitory effect and mechanism of Pichia membranaefaciens composite protein film on postharvest rot of peaches[J]. Food and Fermentation Industries, 2024, 50（5）: 274−279.
[12]	TERAO D, DE LIMA NECHET K, PONTE M S, et al. Physical postharvest treatments combined with antagonistic yeast on the control of orange green mold[J]. Scientia Horticulturae,2017,224:317−323. doi: 10.1016/j.scienta.2017.06.038
[13]	HASSAN H, MOHAMED M T M, YUSOFF S F, et al. Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved[J]. Agronomy,2021,11(4):760. doi: 10.3390/agronomy11040760
[14]	季小诗. 拮抗酵母GS316发酵液对冬枣保鲜效果研究及应用[D]. 天津:天津科技大学, 2021. [JI X S. Study on the effect of antagonistic yeast GS316 fermentation brothon the preservation of winter jujube and its application[D]. Tianjin:Tianjin University of Science and Technology, 2021.] JI X S. Study on the effect of antagonistic yeast GS316 fermentation brothon the preservation of winter jujube and its application[D]. Tianjin: Tianjin University of Science and Technology, 2021.
[15]	BASIAK E, LENART A, DEBEAUFORT F. How glycerol and water contents affect the structural and functional properties of starch-based edible films[J]. Polymers,2018,10(4):412. doi: 10.3390/polym10040412
[16]	张锋. 1-MCP、Ca和限制性气调(MA)处理对采后苹果果实软化的影响[D]. 杨凌:西北农林科技大学, 2009. [ZHANG F. Effects of 1-MCP、Callcium and Modified Atomosphere on apple fruit softening in postharvest[D]. Yangling:Northwest A&F University, 2009.] ZHANG F. Effects of 1-MCP、Callcium and Modified Atomosphere on apple fruit softening in postharvest[D]. Yangling: Northwest A&F University, 2009.
[17]	徐雨晗. 1-甲基环丙烯和乙烯利处理对鲜切荸荠品质的影响及机理探究[D]. 武汉:武汉轻工大学, 2023. [XU Y H. Effects and mechanisms of 1-MCP and ethephon on the quality of fresh-cut Chinese water chestnuts[D]. Wuhan:Wuhan Polytechnic University, 2023.] XU Y H. Effects and mechanisms of 1-MCP and ethephon on the quality of fresh-cut Chinese water chestnuts[D]. Wuhan: Wuhan Polytechnic University, 2023.
[18]	LEE C Y, KAGAN V, JAWORSKI A W, et al. Enzymatic browning in relation to phenolic compounds and polyphenoloxidase activity among various peach cultivars[J]. Journal of Agricultural and Food Chemistry,1990,38(1):99−101. doi: 10.1021/jf00091a019
[19]	曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京:中国轻工业出版社, 2007. [CAO J K, JIANG W B, ZHAO Y M. Experiment guidance of postharvest physiology and biochemistry of fruits and vegetables[M]. Beijing:China Light Industry Press, 2007.] CAO J K, JIANG W B, ZHAO Y M. Experiment guidance of postharvest physiology and biochemistry of fruits and vegetables[M]. Beijing: China Light Industry Press, 2007.
[20]	KARL A D, PECK G M. Greater sunlight exposure during early fruit development increases polyphenol concentration, soluble solid concentration, and fruit mass of cider apples[J]. Horticulturae,2022,8(11):993. doi: 10.3390/horticulturae8110993
[21]	杨昭辉, 王笑笑, 赵祺, 等. 膜醭毕赤酵母菌外泌蛋白抑菌复合膜的制备及性能研究[J]. 食品与发酵工业,2022,48(12):215−219. [YANG Z H, WANG X X, ZHAO Q, et al. Preparation and properties of antibacterial composite membrane of secreted protein from Pichia membranaefaciens[J]. Food and Fermentation Industries,2022,48(12):215−219.] YANG Z H, WANG X X, ZHAO Q, et al. Preparation and properties of antibacterial composite membrane of secreted protein from Pichia membranaefaciens[J]. Food and Fermentation Industries, 2022, 48（12）: 215−219.
[22]	陈莹莹, 张亚敏, 郭红莲. 膜醭毕赤酵母挥发性物质对灰葡萄孢菌的抑菌活性及组分分析[J]. 食品与发酵工业,2023,49(13):64−69. [CHEN Y Y, ZHANG Y M, GUO H L. Antifungal activity of volatile compounds from Pichia membranaefaciens and its component analysis against Botrytis cinerea[J]. Food and Fermentation Industries,2023,49(13):64−69.] CHEN Y Y, ZHANG Y M, GUO H L. Antifungal activity of volatile compounds from Pichia membranaefaciens and its component analysis against Botrytis cinerea[J]. Food and Fermentation Industries, 2023, 49（13）: 64−69.
[23]	刘艺璇, 陈海艺, 徐悦, 等. 膜醭毕赤酵母KD-316-3复合保鲜液的抑菌机制与初步应用[J]. 食品研究与开发,2024,45(12):13−20. [LIU Y X, CHEN H Y, XU Y. Antifungal mechanism and preliminary application of composite preservation liquid containing Pichia membranaefaciens KD-316-3[J]. Food Research and Development,2024,45(12):13−20.] LIU Y X, CHEN H Y, XU Y. Antifungal mechanism and preliminary application of composite preservation liquid containing Pichia membranaefaciens KD-316-3[J]. Food Research and Development, 2024, 45（12）: 13−20.
[24]	胡小莉, 张雪, 张红杰, 等. 纤维性能对纸张水蒸气阻隔性能的影响[J]. 中国造纸,2024,43(1):38−50. [HU X L, ZHANG X, ZHANG H J, et al. Effect of fiber properties on water vapor barrier performance of paper[J]. China Pulp & Paper,2024,43(1):38−50.] doi: 10.11980/j.issn.0254-508X.2024.01.005 HU X L, ZHANG X, ZHANG H J, et al. Effect of fiber properties on water vapor barrier performance of paper[J]. China Pulp & Paper, 2024, 43（1）: 38−50. doi: 10.11980/j.issn.0254-508X.2024.01.005
[25]	RANI H, SINGH S P, YADAV T P, et al. In-vitro catalytic, antimicrobial and antioxidant activities of bioengineered copper quantum dots using Mangifera indica (L.) leaf extract[J]. Materials Chemistry and Physics,2020,239:122052.
[26]	HAN H S, SONG K B. Antioxidant activities of mandarin (Citrus unshiu) peel pectin films containing sage (Salvia officinalis) leaf extract[J]. International Journal of Food Science & Technology,2020,55(9):3173−3181.
[27]	LEI Y L, WU H J, JIAO C, et al. Investigation of the structural and physical properties, antioxidant and antimicrobial activity of pectin-konjac glucomannan composite edible films incorporated with tea polyphenol[J]. Food Hydrocolloids,2019,94:128−135.
[28]	李欣霏. 镍离子诱导的鸡蛋清溶菌酶聚集和纤维化动力学的拉曼光谱研究[D]. 合肥: 中国科学技术大学, 2023. [LI X F. Nickel ion-induced aggregationand amyloid fibrillation kineticsof hen egg white lysozyme by Raman spectroscopy[D]. Hefei: University of Science and Technology of China, 2023.] LI X F. Nickel ion-induced aggregationand amyloid fibrillation kineticsof hen egg white lysozyme by Raman spectroscopy[D]. Hefei: University of Science and Technology of China, 2023.
[29]	BARBA-OSTRIA C, GONZALEZ-PASTOR R, CASTILLO-SOLÍS F, et al. Bioactive properties of microencapsulated anthocyanins from Vaccinium floribundum and Rubus glaucus[J]. Molecules,2024,29:5504.
[30]	孙书境. EGCG的添加对大豆蛋白壳聚糖复合膜的影响[D]. 哈尔滨:东北农业大学, 2022. [SUN S J. Effect of EGCG addition on soybean protein chitosan composite films[D]. Harbin:Northeast Agricultural University, 2022.] SUN S J. Effect of EGCG addition on soybean protein chitosan composite films[D]. Harbin: Northeast Agricultural University, 2022.
[31]	YOSHIYAMA Y, TANAKA K, YOSHIYAMA K, et al. Trehalose accumulation enhances tolerance of Saccharomyces cerevisiae to acetic acid[J]. Journal of Bioscience and Bioengineering,2015,119(2):172−175. doi: 10.1016/j.jbiosc.2014.06.021
[32]	章国华. 生物基涂覆牛皮纸的制备及性能研究[J]. 数字印刷,2020(1):53−58. [ZHANG G H. Preparation and properties of bio-based coated kraft paper[J]. Digital Pinting,2020(1):53−58.] ZHANG G H. Preparation and properties of bio-based coated kraft paper[J]. Digital Pinting, 2020（1）: 53−58.
[33]	XU L N, CAO W H, LI R, et al. Properties of soy protein isolate/nano-silica films and their applications in the preservation of Flammulina velutipes[J]. Journal of Food Processing and Preservation,2019,43(11):e14177.
[34]	张微微. 植物精油聚氨酯抗菌微胶囊的制备及其应用研究[D]. 上海:上海应用技术大学, 2022. [ZHANG W W. Preparation and application of polyurethane coated antimicrobial microcapsules entrapping plant essential oil[D]. Shanghai:Shanghai Institute of Technology, 2022.] ZHANG W W. Preparation and application of polyurethane coated antimicrobial microcapsules entrapping plant essential oil[D]. Shanghai: Shanghai Institute of Technology, 2022.
[35]	贺军花, 马利菁, 周会玲. 根皮苷对苹果采后灰霉病的影响[J]. 食品科学,2018,39(15):190−196. [HE J H, MA L J, ZHOU H L. Effect of Phlorizin treatment on postharvest gray mold of apples[J]. Food Science,2018,39(15):190−196.] doi: 10.7506/spkx1002-6630-201815028 HE J H, MA L J, ZHOU H L. Effect of Phlorizin treatment on postharvest gray mold of apples[J]. Food Science, 2018, 39（15）: 190−196. doi: 10.7506/spkx1002-6630-201815028
[36]	WANG Y, LI R, LU R, et al. Preparation of Chitosan/Corn Starch/Cinnamaldehyde films for strawberry preservation[J]. Foods,2019,8(9):423. doi: 10.3390/foods8090423
[37]	SUI X, MENG Z, DONG T T, et al. Enzymatic browning and polyphenol oxidase control strategies[J]. Current Opinion in Biotechnology,2023,81:102921. doi: 10.1016/j.copbio.2023.102921
[38]	GAO K, ZHOU L Y, BI J F, et al. Evaluation of browning ratio in an image analysis of apple slices at different stages of instant controlled pressure drop-assisted hot-air drying (AD-DIC)[J]. Journal of the Science of Food and Agriculture,2017,97(8):2533−2540. doi: 10.1002/jsfa.8070
[39]	ZHANG Z Q, LIU T, XU Y, et al. Sodium pyrosulfite inhibits the pathogenicity of Botrytis cinerea by interfering with antioxidant system and sulfur metabolism pathway[J]. Postharvest Biology and Technology,2022,189:28−36.
[40]	MDITSHWA A, MAGWAZA L S, TESFAY S Z, et al. Postharvest factors affecting vitamin C content of citrus fruits:A review[J]. Scientia Horticulturae,2017,218:95−104. doi: 10.1016/j.scienta.2017.02.024

Supplements (0)

Get Citation

PDF

XML

Article Metrics

Article views (30) PDF downloads (7)

Science and Technology of Food Industry

Preparation of Composite Preservation Paper containing Antagonistic Fungi and Its Application on Apple Preservation

Abstract

References

Catalog

Article Metrics

Export File

Citation

Format

Content