Sustained Release Properties of Acid Resistant Carboxymethyl Chitosan Gel Microspheres Loaded with Anthocyanins

LI Xu; CHU Ruoqing; LIU Xinru; LIU Changxia; YE Xia; FAN Xiaozhen

doi:10.13386/j.issn1002-0306.2024020076

Volume 45 Issue 15

Jul. 2024

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Science and Technology of Food Industry > 2024 > 45(15): 152-160. > DOI: 10.13386/j.issn1002-0306.2024020076

LI Xu, CHU Ruoqing, LIU Xinru, et al. Sustained Release Properties of Acid Resistant Carboxymethyl Chitosan Gel Microspheres Loaded with Anthocyanins[J]. Science and Technology of Food Industry, 2024, 45(15): 152−160. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020076.

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Sustained Release Properties of Acid Resistant Carboxymethyl Chitosan Gel Microspheres Loaded with Anthocyanins

Department of Chemistry and Chemical Engineering, Cangzhou Normal University, Cangzhou 061000, China

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Received Date: February 18, 2024
Available Online: June 04, 2024

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Abstract

Abstract

This study was aimed at broadening the application range of chitosan hydrogels and constructing an edible anthocyanins (ACNs) delivery system. The acid resistant carboxymethyl chitosan (CMCS) gel microspheres (ACNs/CMCS-SA) loaded with ACNs were prepared by inversion-phase emulsion method. During the process, corn oil was used as continuous phase and salicylaldehyde (SA) was used as crosslinking agent. The structure and morphology of ACNs/CMCS-SA were characterized. And the stability, swelling ability and sustained release properties were studied. The results showed that ACNs/CMCS-SA had a micron particle size with smooth and round surface. The gel microspheres showed good acid resistance and pH responsiveness during swelling and sustained release experiments. The sustained release behavior of ACNs conformed to Weibull model. In pH7 and pH9 media, the release process of ACNs was controlled by a combination mechanism of diffusion and skeleton dissolution. In pH3 and 5 media, it was controlled by a combination of Fick diffusion and Case Ⅱ transport (order 0 grade-swelling dependent release). The preparation method of ACNs/CMCS-SA was simple and green, and the sample exhibited good gastric acid protection and intestinal release performance for ACNs. Therefore, this study lays a theoretical and experimental foundation for the development of environmentally friendly drug encapsulation materials and the application of ACNs.
- anthocyanins (ACNs),
- release properties,
- dynamics,
- pH response,
- inversion-phase emulsion method,
- gel microsphere

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References

[1]	CHEN L Z, ZHONG J J, LIN Y Y, et al. Microwave and enzyme co-assisted extraction of anthocyanins from purple-heart radish:Process optimization, composition analysis and antioxidant activity[J]. LWT,2023,187:115312. doi: 10.1016/j.lwt.2023.115312
[2]	李煦, 白雪晴, 刘长霞, 等. 天然花青素的抗氧化机制及功能活性研究进展[J]. 食品安全质量检测学报,2021,12(20):8163−8171. [LI X, BAI X Q, LIU C X, et al. Research progress on antioxidant mechanism and functional activity of natural anthocyanins[J]. Journal of Food Safety and Quality,2021,12(20):8163−8171.] LI X, BAI X Q, LIU C X, et al. Research progress on antioxidant mechanism and functional activity of natural anthocyanins[J]. Journal of Food Safety and Quality, 2021, 12（20）: 8163−8171.
[3]	FARIA A, PESTANA D, AZEVEDO J, et al. Absorption of anthocyanins through intestinal epithelial cells-putative involvement of GLUT₂[J]. Molecular Nutrition & Food Research,2009,53(11):1430−1437.
[4]	HUANG Y X, ZHOU S Y, ZHAO G H, et al. Destabilisation and stabilisation of anthocyanins in purple-fleshed sweet potatoes:A review[J]. Trends in Food Science & Technology,2021,116:1141−1154.
[5]	YUAN Y T, FAN Q, XU X Y, et al. Nanocarriers based on polysaccharides for improving the stability and bioavailability of anthocyanins:A review[J]. Carbohydrate Polymer Technologies and Applications,2023,6:100346. doi: 10.1016/j.carpta.2023.100346
[6]	HE J, MAGNUSON B A, LALA G, et al. Intact anthocyanins and metabolites in rat urine and plasma after 3 months of anthocyanin supplementation[J]. Nutrition and Cancer,2006,54(1):3−12. doi: 10.1207/s15327914nc5401_2
[7]	ZHOU X, NIE S, LIU L, et al. Compound hydrogels derived from gelatin and gellan gum regulates the release of anthocyanins in simulated digestion[J]. Food Hydrocolloids,2022,127:107487. doi: 10.1016/j.foodhyd.2022.107487
[8]	GHREAGHAJLOU N, HALLAJ-NEZHADI S, GHASEMPOUR Z. Nano-liposomal system based on lyophilization of monophase solution technique for encapsulating anthocyanin-rich extract from red cabbage[J]. Dyes Pigments,2022,202:110263. doi: 10.1016/j.dyepig.2022.110263
[9]	THIECLA K O R, SILVA M P D, LOURENO F R, et al. Nanoencapsulation of anthocyanins from blackberry (Rubus spp) through pectin and lysozyme self-assembling[J]. Food Hydrocolloids,2021,114:106563. doi: 10.1016/j.foodhyd.2020.106563
[10]	IRMAK O S, NESLIHAN A D, KUBRA U, et al. Lyophilized nano-liposomal system for red onion (Allium cepa L.) peel anthocyanin:Characterization, bioaccessibility and release kinetics[J]. Food Bioscience,2023,53:102702. doi: 10.1016/j.fbio.2023.102702
[11]	SANTIAGOGARCÍA A P, LEÓNMARTÍNEZ M F, GUTIÉRREZ C M, et al. Microencapsulation of strawberry juice in Agave angustifolia fructans:Effect of spray-drying conditions on the anthocyanin content and physicochemical properties[J]. International Journal of Food Science Technology,2023,58(12):6725−6735. doi: 10.1111/ijfs.16529
[12]	LIU R R, WANG X H, YANG L X, et al. Coordinated encapsulation by β-cyclodextrin and chitosan derivatives improves the stability of anthocyanins[J]. International Journal of Biological Macromolecules,2023,242:125060. doi: 10.1016/j.ijbiomac.2023.125060
[13]	WU C L, JULIAN M D, MA B H, et al. Composite hydrogels formed from okara cellulose nanofibers and carrageenan:Fabrication and characterization[J]. International Journal of Biological Macromolecules,2023,258(P2):129079.
[14]	YING J. Synthesis of porous starch microgels for the encapsulation, delivery and stabilization of anthocyanins[J]. Journal of Food Engineering,2021,302:110552. doi: 10.1016/j.jfoodeng.2021.110552
[15]	LI W J, LINLI F Z, YANG W Y, et al. Enhancing the stability of natural anthocyanins against environmental stressors through encapsulation with synthetic peptide-based gels[J]. International Journal of Biological Macromolecules,2023,253:127133. doi: 10.1016/j.ijbiomac.2023.127133
[16]	曹亚婵, 刘晓坤, 党奇峰, 等. 基于壳聚糖的抗菌可注射自愈性水凝胶的制备及其生物相容性研究[J]. 中国海洋大学学报(自然科学版),2024,54(3):60−69. [CAO Y C, LIU X K, DANG Q F, et al. Preparation and biocompatibility of antibacterial injectable chitosan-based hydrogel for self-healing biomateria[J]. Periodical of Ocean University of China,2024,54(3):60−69.] CAO Y C, LIU X K, DANG Q F, et al. Preparation and biocompatibility of antibacterial injectable chitosan-based hydrogel for self-healing biomateria[J]. Periodical of Ocean University of China, 2024, 54（3）: 60−69.
[17]	李煦, 董翠芳, 刘长霞, 等. 负载花色苷的壳聚糖-水杨醛水凝胶的制备及性能[J]. 食品工业科技,2023,44(9):111−118. [LI X, DONG C F, LIU C X, et al. Preparation and properties of chitosan salicylaldehyde hydrogel loaded with anthocyanins[J]. Science and Technology of Food Industry,2023,44(9):111−118.] LI X, DONG C F, LIU C X, et al. Preparation and properties of chitosan salicylaldehyde hydrogel loaded with anthocyanins[J]. Science and Technology of Food Industry, 2023, 44（9）: 111−118.
[18]	LIU C X, DONG C F, LIU S H, et al. Multiple chiroptical switches and logic circuit based on salicyl- imine- chitosan hydrogel[J]. Carbohydrate Polymers,2021,257:117534. doi: 10.1016/j.carbpol.2020.117534
[19]	FATEMEH K, SAEED S S. Synthesis and characterization of a novel hydrogel based on carboxymethyl chitosan/sodium alginate with the ability to release simvastatin for chronic wound healing[J]. Biomedial Materials,2023,18(2):025001. doi: 10.1088/1748-605X/acb0a3
[20]	GUO F B, LIU Y, CHEN S Q, et al. A schiff base hydrogel dressing loading extracts from Periplaneta americana for diabetic wound healing[J]. International Journal of Biological Macromolecules,2023,230:123256. doi: 10.1016/j.ijbiomac.2023.123256
[21]	YU R, ZHANG Y, BARBORU M, et al. Biobased pH-responsive and self-healing hydrogels prepared from O-carboxymethyl chitosan and a 3-dimensional dynamer as cartilage engineering scaffold[J]. Carbohydrate Polymers,2020,244:116471. doi: 10.1016/j.carbpol.2020.116471
[22]	李俊杰, 贾鹏, 刘功稳, 等. 一种口服骨靶向微/纳水凝胶微球制备及对雌性去势小鼠骨质量影响的研究[J]. 中国骨质疏松杂志,2023,29(11):1581−1586,1597. [LI J J, JIA P, LIU G W, et al. Preparation of an oral bone-targeting micro/nano hydrogel microsphere and its effect on bone quality in ovariectomized mice[J]. Chinese Journal of Osteoporosis,2023,29(11):1581−1586,1597.] doi: 10.3969/j.issn.1006-7108.2023.11.005 LI J J, JIA P, LIU G W, et al. Preparation of an oral bone-targeting micro/nano hydrogel microsphere and its effect on bone quality in ovariectomized mice[J]. Chinese Journal of Osteoporosis, 2023, 29（11）: 1581−1586,1597. doi: 10.3969/j.issn.1006-7108.2023.11.005
[23]	GIULBUDAGIAN M, YEALLAND G, HNZHE S, et al. Breaking the barrier-Potent anti-inflammatory activity following efficient topical delivery of etanercept using thermoresponsive nanogels[J]. Theranostics,2018,8(2):450−463. doi: 10.7150/thno.21668
[24]	ZHU C, WANG S, WANG D, et al. Novel nano-micro-macro multiple-nested hydrogel with gradient ciliary neurotrophic factor distribution induces directional axon regeneration of retinal ganglion cells[J]. Colloids and Surfaces-A Physicochemical and Engineering Aspects,2023,675:131904. doi: 10.1016/j.colsurfa.2023.131904
[25]	LI X, WU X L. The microspheres/hydrogels scaffolds based on the proteins, nucleic acids, or polysaccharides composite as carriers for tissue repair:A review[J]. International Journal of Biological Macromolecules,2023,253:126611. doi: 10.1016/j.ijbiomac.2023.126611
[26]	SATOMI T, ANDREA C, SUZUKA S, et al. Preparation of ultrasmall cyclodextrin nanogels by an inverse emulsion method using a cationic surfactant[J]. Chemical Communications,2023,59(27):4071−4074. doi: 10.1039/D3CC00523B
[27]	刘长姣, 郑霞, 熊湘炜, 等. 分光光度法测定黑米花青素方法的建立[J]. 粮食与油脂,2019,32(1):73−77. [LIU C J, ZHENG X, XIONG X W, et al. Establishment of a spectrophotometric method for the determination of anthocyanins in black rice[J]. Food and Oil,2019,32(1):73−77.] doi: 10.3969/j.issn.1008-9578.2019.01.020 LIU C J, ZHENG X, XIONG X W, et al. Establishment of a spectrophotometric method for the determination of anthocyanins in black rice[J]. Food and Oil, 2019, 32（1）: 73−77. doi: 10.3969/j.issn.1008-9578.2019.01.020
[28]	戴文, 王晓东, 黄培, 等. 乳液模板法制备聚酰亚胺中空微球及其形貌调控[J]. 高分子材料科学与工程,2023,39(7):25−32. [DAI W, WANG X D, HUANG P, et al. Morphology control and preparation of polyimide hollow microspheres by emulsion template method[J]. Polymer Materials Science & Engineering,2023,39(7):25−32.] DAI W, WANG X D, HUANG P, et al. Morphology control and preparation of polyimide hollow microspheres by emulsion template method[J]. Polymer Materials Science & Engineering, 2023, 39（7）: 25−32.
[29]	ZHANG B, WANG Q, ZHOU P P, et al. Copigmentation evidence of oenin with phenolic compounds:A comparative study of spectrographic, thermodynamic and theoretical data[J]. Food Chemistry,2020,313:126163. doi: 10.1016/j.foodchem.2020.126163
[30]	薛宏坤, 李鹏程, 钟雪, 等. 高速逆流色谱分离纯化桑葚花色苷及其抗氧化活性[J]. 食品科学,2020,41(15):96−104. [XUE H S, LI P C, ZHONG X, et al. Separation and purification of anthocyanins from mulberry fruit by high-speed counter-current chromatography and their antioxidant activity[J]. Food Science,2020,41(15):96−104.] doi: 10.7506/spkx1002-6630-20190715-193 XUE H S, LI P C, ZHONG X, et al. Separation and purification of anthocyanins from mulberry fruit by high-speed counter-current chromatography and their antioxidant activity[J]. Food Science, 2020, 41（15）: 96−104. doi: 10.7506/spkx1002-6630-20190715-193
[31]	WU X H, LIN Q W, BELWAL T, et al. Effect of advanced/hybrid oxidation process involving ultrasonication and ultraviolet radiation (sonophotolysis) on anthocyanin stability:Degradation kinetics and mechanism[J]. Food Chemistry,2022,370:131083. doi: 10.1016/j.foodchem.2021.131083
[32]	KOSMIDIS K, MACHERAS P. On the dilemma of fractal or fractional kinetics in drug release studies:A comparison between Weibull and Mittag-Leffler functions[J]. International Journal of Pharmaceutics,2018,543(1-2):269−273. doi: 10.1016/j.ijpharm.2018.03.060
[33]	PAPADOPOILOU V, KOSMIDIS K, VLACHOU M, et al. On the use of the Weibull function for the discernment of drug release mechanisms[J]. International Journal of Pharmaceutics,2006,309(1−2):44−50. doi: 10.1016/j.ijpharm.2005.10.044
[34]	NAZIM N, STEFAN K. Fundamental advances in hydrogels for the development of the next generation of smart delivery systems as biopharmaceuticals[J]. International Journal of Pharmaceutics,2023,633:122634. doi: 10.1016/j.ijpharm.2023.122634
[35]	李凌冰, 谭业邦. 亲水聚合物凝胶系统中药物控制释放两类特殊情况的数学模型[J]. 生物医学工程学杂志,2003,20(1):17−21. [LI L B, TAN Y B. Two sorts of problems on drug controlled release from swellable polymer[J]. Journal of Biomedical Engineering,2003,20(1):17−21.] doi: 10.3321/j.issn:1001-5515.2003.01.006 LI L B, TAN Y B. Two sorts of problems on drug controlled release from swellable polymer[J]. Journal of Biomedical Engineering, 2003, 20（1）: 17−21. doi: 10.3321/j.issn:1001-5515.2003.01.006
[36]	LIU L Y, ZHANG D D, SONG X X, et al. Compound hydrogels derived from gelatin and gellan gum regulates the release of anthocyanins in simulated digestion[J]. Food Hydrocolloids,2022,127(6):107487.
[37]	GHAZAL S, KORDESTANI S S, TAHRIRI M, et al. Evaluation of L929 cell morphology on anthocyanin-containing gelatin-based hydrogel for early detection of infection[J]. Bio-Design and Manufacturing,2019,2(3):181−186. doi: 10.1007/s42242-019-00047-6

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Science and Technology of Food Industry

Sustained Release Properties of Acid Resistant Carboxymethyl Chitosan Gel Microspheres Loaded with Anthocyanins

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