Screening of Selenium-enriched Yeasts with Enhanced Aroma and High Alcohol Production by ARTP Mutagenesis

WANG Guoqin; REN Yizhuo; ZHANG Jie; MA Xian; ZHENG Rui; SU Jianyu; YU Junwei; YUE Sijun

doi:10.13386/j.issn1002-0306.2023110161

Volume 45 Issue 18

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(18): 146-156. > DOI: 10.13386/j.issn1002-0306.2023110161

WANG Guoqin, REN Yizhuo, ZHANG Jie, et al. Screening of Selenium-enriched Yeasts with Enhanced Aroma and High Alcohol Production by ARTP Mutagenesis[J]. Science and Technology of Food Industry, 2024, 45(18): 146−156. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110161.

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Screening of Selenium-enriched Yeasts with Enhanced Aroma and High Alcohol Production by ARTP Mutagenesis

1.
College of Life Sciences, Ningxai University, Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Eestern China, Yinchuan 750021, China
2.
Ningxia Zhongning Wolfberry Industry Innovation Research Institute Co., Ltd., Yinchuan 755100, China

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Received Date: November 15, 2023
Available Online: August 06, 2024

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Abstract

Abstract

In order to obtain endophytic selenium-rich yeast from Lycium barbarum with strong ability producing alcohol and aroma, and then promote the selenium-rich fermentation products and improve the flavor, this study took the endophytic yeast of L. barbarum stored in the laboratory as experimental materials, and the yeast with strong selenium-rich ability was obtained through the initial screening of selenium-resistant method and red selenium methods. Atmospheric room temperature plasma (ARTP) technology was used to obtain mutant strains and through TTC medium, Duchenne tubule fermentation, alcohol production, ester and acid production, tolerance and other experiments to screen out the target strains of aroma enhancement and high alcohol production. The content of selenium in yeast cell and fermentation liquid was determined by hydride generation atomic fluorescence spectrometry. The results showed that M1 Saccharomyces cerevisiae and M16 Clavispora lusitaniae were obtained by primary selenium screening, the mutagenic strain M1-5 of S. cerevisiae with higher alcohol production capacity and the mutagenic strain M16-28 of C. lusitaniae with higher aroma production capacity were obtained through ARTP mutagenesis. The alcohol production capacity of M1-5 increased by 38.07% compared with M1, and the ester production capacity of M16-28 increased by 107.62% compared with M16, and the genetic stability was good. The selenium contents in the intracellular and fermentation broth of the M1-5 strains were 204.8 and 1158 μg/L, respectively, and in M16-28 were 666.45μg/L and 1830 μg/L, respectively. Therefore, the selenium enrichment capacity of M16-28 was higher than that of M1-5, and the selenium content of fermentation liquid was extremely significantly higher than that of yeast cells (P<0.001). In conclusion, the selenium-rich yeast with mutagenic strain M1-5 producing alcohol and M16-28 producing aroma were screened out, which had excellent fermentation characteristics and potential application prospects in the development and mining of fermented food and selenium-rich functional products.
- Lycium barbarum,
- endophytic yeast,
- selenium-rich,
- ARTP,
- high alcohol production,
- aroma enhancement,
- fermentation characteristics.

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References

[1]	ZHU H Q, CHEN S Q, LI R B, et al. Selenium-rich yeast counteracts the inhibitory effect of nanoaluminum on the formation of porcine neutrophil extracellular traps[J]. Research in Veterinary Science,2023,161:138−144. doi: 10.1016/j.rvsc.2023.06.018
[2]	CAO C Y, LI X W, QIN L, et al. High Selenium Yeast mitigates aluminum-induced cerebral inflammation by increasing oxidative stress and blocking NO production[J]. Biometals,2018,31(5):835−843. doi: 10.1007/s10534-018-0128-0
[3]	涂青, 杨双全, 章之柱, 等. 富硒酵母发酵工艺的优化[J]. 中国酿造,2022,41(10):140−145. [TU Q, YANG S Q, ZHANG Z Z, et al. Optimization of fermentation process of selenium-rich yeast[J]. China Brewing,2022,41(10):140−145.] TU Q, YANG S Q, ZHANG Z Z, et al. Optimization of fermentation process of selenium-rich yeast[J]. China Brewing, 2022, 41（10）: 140−145.
[4]	SUN N, DANG H, ZHANG Y Y, et al. Inorganic Selenium Transformation into Organic Selenium by Monascus purpureus[J]. Foods,2023,12(18):3375. doi: 10.3390/foods12183375
[5]	LIAO X L, RAO S, YU T, et al. Selenium yeast promoted the Se accumulation, nutrient quality and antioxidant system of cabbage (Brassica oleracea var. capitata L.)[J]. Plant Signal Behav,2021,16(6):1907042. doi: 10.1080/15592324.2021.1907042
[6]	王凤梅, 张邦建, 岳泰新. 内蒙古西部地区野生葡萄酒相关酵母发酵及产酯能力分析[J]. 南方农业学报,2019,50(4):825−830. [WANG F M, ZHANG B J, YUE T X. The ability of fermentation and esters production of wild winemaking yeasts isolated from the western district of Inner Mongolia[J]. Acta Agriculturae Sinica of Southern China,2019,50(4):825−830.] doi: 10.3969/j.issn.2095-1191.2019.04.20 WANG F M, ZHANG B J, YUE T X. The ability of fermentation and esters production of wild winemaking yeasts isolated from the western district of Inner Mongolia[J]. Acta Agriculturae Sinica of Southern China, 2019, 50（4）: 825−830. doi: 10.3969/j.issn.2095-1191.2019.04.20
[7]	郑超群. 基于酿酒酵母和异常汉逊酵母混合发酵技术的黄酒生产工艺及风味成分研究[D]. 杭州:浙江工商大学, 2023. [ZHENG C Q. Research on the production process and flavor components of rice wine based on the mixed fermentation technology of Saccharomyces cerevisiae and Hansenula anomalies[D].Hangzhou: Zhejiang Gongshang University, 2023.] ZHENG C Q. Research on the production process and flavor components of rice wine based on the mixed fermentation technology of Saccharomyces cerevisiae and Hansenula anomalies[D].Hangzhou: Zhejiang Gongshang University, 2023.
[8]	王静尧. 高产酯非酿酒酵母筛选及在混菌发酵中的发酵特性研究[D]. 阿拉尔:塔里木大学, 2023. [WANG J Y. Screening of non-Saccharomyces cerevisiae with high ester yield and its fermentation characteristics in mixed fermentation[D]. Alaer:Tarim University, 2023.] WANG J Y. Screening of non-Saccharomyces cerevisiae with high ester yield and its fermentation characteristics in mixed fermentation[D]. Alaer: Tarim University, 2023.
[9]	李秀婷, 范光森, 孙宝国, 等. 产己酸乙酯的葡萄牙棒孢酵母菌株及其培养方法与应用:中国,111909861A[P]. 2020-11-10. [LI X T, FAN G S, SUN B G, et al. Strain of Clavispora lusitaniae producing ethyl caproate and its culture method and application:China, 111909861A[P]. 2020-11-10.] LI X T, FAN G S, SUN B G, et al. Strain of Clavispora lusitaniae producing ethyl caproate and its culture method and application: China, 111909861A[P]. 2020-11-10.
[10]	LIU Y, CHEN X, WEI D, et al. Breeding a novel chlorophyll-deficient mutant of Auxenochlorella pyrenoidosa for high-quality protein production by atmospheric room temperature plasma mutagenesis[J]. Bioresour Technol,2023,390:129907. doi: 10.1016/j.biortech.2023.129907
[11]	ZHANG Q, MIAO R Y, FENG R C, et al. Application of Atmospheric and room-temperature plasma (ARTP) to microbial breeding[J]. Current Issues in Molecular Biology,2023,45(8):6466−6484. doi: 10.3390/cimb45080408
[12]	汪锡武, 冯婉婷, 方旭娟, 等. 富硒啤酒酵母的选育及有机硒含量的测定[J]. 食品与发酵工业,2024,50(10):48−54. [WANG X W, FENG W T, FANG X J, et al. Breeding of selenium-rich beer yeast and determination of organic selenium content[J]. Food and Fermentation Industry,2024,50(10):48−54.] WANG X W, FENG W T, FANG X J, et al. Breeding of selenium-rich beer yeast and determination of organic selenium content[J]. Food and Fermentation Industry, 2024, 50（10）: 48−54.
[13]	林仙菊, 张祝兰, 严凌斌, 等. ARTP诱变选育环孢菌素A高产菌株及发酵工艺优化[J]. 工业微生物,2023,53(4):158−163. [LIN X J, ZHANG Z L, YAN L B, et al. Study on screening of high-yield cyclosporin A producing strain by atmospheric and room temperature plasma mutagenesis and optimization of fermentation conditions[J]. Industrial Microbiology,2023,53(4):158−163.] doi: 10.3969/j.issn.1001-6678.2023.04.044 LIN X J, ZHANG Z L, YAN L B, et al. Study on screening of high-yield cyclosporin A producing strain by atmospheric and room temperature plasma mutagenesis and optimization of fermentation conditions[J]. Industrial Microbiology, 2023, 53（4）: 158−163. doi: 10.3969/j.issn.1001-6678.2023.04.044
[14]	HONG K Q, FU X M, LEI F F, et al. Selection of salt-tolerance and ester-producing mutant Saccharomyces cerevisiae to improve flavour formation of soy sauce during co-fermentation with torulopsis globosa[J]. Foods,2023,12(18):3449. doi: 10.3390/foods12183449
[15]	杨新, 陈莉, 杨双全, 等. 开阳地区桑葚果园富硒酵母菌的分离、筛选及鉴定[J]. 食品与发酵工业,2020,46(24):66−73. [YANG X, CHEN L, YANG S Q, et al. Isolation, screening and identification of selenium-enrichedyeast from mulberry orchard of Kaiyang[J]. Food and Fermentation Industry,2020,46(24):66−73.] YANG X, CHEN L, YANG S Q, et al. Isolation, screening and identification of selenium-enrichedyeast from mulberry orchard of Kaiyang[J]. Food and Fermentation Industry, 2020, 46（24）: 66−73.
[16]	刘程, 梁静一美, 王周利, 等. 茯砖茶中冠突散囊菌的分离鉴定及富硒菌株筛选[J]. 西北农业学报,2022,31(10):1344−1356. [LIU C, LIANG JING Y M, WANG Z L, et al. Isolation and identification of eurotium cristatum in Fucha tea and screening of selenium-rich strains[J]. Journal of Northwest Agriculture,2022,31(10):1344−1356.] LIU C, LIANG JING Y M, WANG Z L, et al. Isolation and identification of eurotium cristatum in Fucha tea and screening of selenium-rich strains[J]. Journal of Northwest Agriculture, 2022, 31（10）: 1344−1356.
[17]	胡惠影. 乳杆菌(Lactobacillus sp.)JZ07富集转化硒的特性研究[D]. 郑州:河南工业大学, 2018. [HU H Y. Characteristics of Selenium accumulation and transformation by Lactobacillus sp. JZ07[D]. Zhengzhou:Henan University of Technology, 2018.] HU H Y. Characteristics of Selenium accumulation and transformation by Lactobacillus sp. JZ07[D]. Zhengzhou: Henan University of Technology, 2018.
[18]	中华人民共和国国家监督检验检疫总局. GB/T 5009.93-2017食品安全国家标准食品中硒的测定[S]. 北京:中国标准出版社,2017. [General Administration of Supervision, Inspection and Quarantine of the People's Republic of China. Determination of selenium in GB/T 5009.93-2017 National standard for food safety. Determination of selenium in food[S]. Beijing:China Standards Press,2017.] General Administration of Supervision, Inspection and Quarantine of the People's Republic of China. Determination of selenium in GB/T 5009.93-2017 National standard for food safety. Determination of selenium in food[S]. Beijing: China Standards Press, 2017.
[19]	金海炎. 猕猴桃酒优良酿酒菌株的选育及混菌发酵工艺优化研究[D].南阳: 南阳师范学院, 2023. [JIN H Y. Study on yeast screening of kiwifruit wine and optimization of its fermentation conditions for compound barm[D]. Nanyang:Nanyang Normal University, 2023.] JIN H Y. Study on yeast screening of kiwifruit wine and optimization of its fermentation conditions for compound barm[D]. Nanyang: Nanyang Normal University, 2023.
[20]	王伟雄. 基于Kluyveromyces marxianus与酿酒酵母混合发酵葡萄酒动力学分析及质量提升研究[D]. 乌鲁木齐:新疆农业大学,2022. [WANG W X. DynamicAnalysis and Quality Improvement of Mixed Fermentation Wine Based on Kluyveromyces Marxianus and Saccharomyces cerevisiae[D]. Urumqi:Xinjiang Agricultural University,2022.] WANG W X. DynamicAnalysis and Quality Improvement of Mixed Fermentation Wine Based on Kluyveromyces Marxianus and Saccharomyces cerevisiae[D]. Urumqi: Xinjiang Agricultural University, 2022.
[21]	中华人民共和国国家监督检验检疫总局. GB/T 5009.225-2016食品安全国家标准酒中乙醇浓度的测定[S]. 北京:中国标准出版社,2016. [General Administration of Supervision, Inspection and Quarantine of the People's Republic of China. GB/T 5009.225-2016 National standard for food safety. Determination of ethanol concentration in alcohol[S]. Beijing:China Standard Press,2016.] General Administration of Supervision, Inspection and Quarantine of the People's Republic of China. GB/T 5009.225-2016 National standard for food safety. Determination of ethanol concentration in alcohol[S]. Beijing: China Standard Press, 2016.
[22]	凌思雨, 王洲, 张会敏, 等. 常压室温等离子体诱变与微生物微液滴培养选育谷胱甘肽高产菌株[J]. 食品科学,2023,44(4):200−208. [LING S Y, WANG Z, ZHANG H M, et al. Breeding of a high-yield glutathione-producing strain by atmospheric and room temperature plasma mutagenesis and microbial microdroplet culture system[J]. Food Science,2023,44(4):200−208.] LING S Y, WANG Z, ZHANG H M, et al. Breeding of a high-yield glutathione-producing strain by atmospheric and room temperature plasma mutagenesis and microbial microdroplet culture system[J]. Food Science, 2023, 44（4）: 200−208.
[23]	杨昳津, 俞剑燊, 夏永军, 等. 优良黄酒酵母的筛选及其抗逆性能分析[J]. 现代食品科技,2015,31(8):261−267. [YANG Y J, YU J S, XIA Y J, et al. Screening for Quality Strains of Yeast from Yellow Rice Wine and Stress Resistance Analysis[J]. Modern Food Science and Technology,2015,31(8):261−267.] YANG Y J, YU J S, XIA Y J, et al. Screening for Quality Strains of Yeast from Yellow Rice Wine and Stress Resistance Analysis[J]. Modern Food Science and Technology, 2015, 31（8）: 261−267.
[24]	LI Y C, RAO J W, MENG F B, et al. Combination of mutagenesis and adaptive evolution to engineer salt-tolerant and aroma-producing yeast for soy sauce fermentation[J]. Journal of the Science of Food and Agriculture,2021,101(10):4288−4297. doi: 10.1002/jsfa.11068
[25]	TIAN T T, WU D H, CHAN-TAT NG, et al. A multiple-step strategy for screening Saccharomyces cerevisiae strains with improved acid tolerance and aroma profiles[J]. Applied Microbiology and Biotechnology,2020,104(7):3097−3107. doi: 10.1007/s00253-020-10451-z
[26]	LIU C F, LI Q, NIU C D, et al. The use of atmospheric and room temperature plasma mutagenesis to create a brewing yeast with reduced acetaldehyde production[J]. Journal of the Institute of Brewing,2018,124:236−243. doi: 10.1002/jib.498
[27]	WEI F Q, LI M L, WANG M, et al. A C6/C5 co-fermenting Saccharomyces cerevisiae strain with the alleviation of antagonism between xylose utilization and robustness[J]. GCB Bioenergy,2020,13:83−97.
[28]	王犁烨, 王浩臣, 马珊, 等. 常压室温等离子体选育高产酒精及酸的酿酒酵母[J]. 食品与机械,2019,35(5):26−31. [WANG L Y, WANG H C, MA S, et al. Breeding of acid-tolerant Saccharomyces cerevisiae strains by ARTP mutation[J]. Food and Machinery,2019,35(5):26−31.] WANG L Y, WANG H C, MA S, et al. Breeding of acid-tolerant Saccharomyces cerevisiae strains by ARTP mutation[J]. Food and Machinery, 2019, 35（5）: 26−31.
[29]	蔡飞, 高飞飞, 王斌, 等. 富硒酵母的分离鉴定及富硒条件的优化[J]. 中国酿造,2018,37(6):103−108. [CAI F, GAO F F, WANG B, et al. Isolation and identification of selenium-enriched yeast and optimization of selenium-enriched conditions[J]. China Brewing,2018,37(6):103−108.] CAI F, GAO F F, WANG B, et al. Isolation and identification of selenium-enriched yeast and optimization of selenium-enriched conditions[J]. China Brewing, 2018, 37（6）: 103−108.
[30]	凡敏, 黄珍, 李欣, 等. 富硒酵母的筛选鉴定及其作为鱼饲料的应用研究[J]. 湖北农业科学,2017,56(8):1416−1419. [FAN M, HUANG Z, LI X, et al. Isolation, identification and application research of fish feed of Selenium-rich yeast[J]. Hubei Agricultural Sciences,2017,56(8):1416−1419.] FAN M, HUANG Z, LI X, et al. Isolation, identification and application research of fish feed of Selenium-rich yeast[J]. Hubei Agricultural Sciences, 2017, 56（8）: 1416−1419.

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Science and Technology of Food Industry

Screening of Selenium-enriched Yeasts with Enhanced Aroma and High Alcohol Production by ARTP Mutagenesis

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