Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from <i>Phellinus igniarius</i>

FU Tingwei; ZHOU Ziwei; SHAO Ying; ZHANG Peng; NING Xiaoya; YANG Yang; CHEN Anhui

doi:10.13386/j.issn1002-0306.2023050290

Volume 45 Issue 7

Mar. 2024

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Science and Technology of Food Industry > 2024 > 45(7): 191-200. > DOI: 10.13386/j.issn1002-0306.2023050290

FU Tingwei, ZHOU Ziwei, SHAO Ying, et al. Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius[J]. Science and Technology of Food Industry, 2024, 45(7): 191−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050290.

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Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius

FU Tingwei^1,,
ZHOU Ziwei¹,
SHAO Ying^{1, 2, ,},
ZHANG Peng³,
NING Xiaoya¹,
YANG Yang¹,
CHEN Anhui^{1, 2}

1.
College of Food (Biological) Engineering, Xuzhou University of Technology, Xuzhou 221111, China
2.
Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou 221111, China
3.
Xuzhou Tianyi Food Research Institute Company Limited, Xuzhou 210009, China

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Received Date: May 28, 2023
Available Online: January 23, 2024

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Abstract

Abstract

In order to study the extraction technology of total flavonoids from Phellinus igniarius systematically, clarify the composition and content of flavonoids in total flavonoids, and explore the biological activity of total flavonoids from Phellinus igniarius. The extraction technology of Phellinus igniarius total flavonoids was optimized by response surface method, the composition of Phellinus igniarius total flavonoids was analyzed, and some of its biological activities were also detected. The results showed that the optimal extraction conditions were extraction temperature of 73 ℃, solid-liquid ratio of 1:50 g/mL and extraction time of 3 h, under which the yield of Phellinus igniarius total flavonoids reached 2.68%. The types and contents of flavonoids in Phellinus igniarius total flavonoids were determined by HPLC. Total flavonoids of Phellinus igniarius mainly contained taxifolin, quercetin and kaempferol, and the content of taxifolin was 3727.31 mg/kg. The analysis of biological activities of Phellinus igniarius total flavonoids showed that it had certain antioxidant, lipid-lowering and hypoglycemic activities in vitro, and the scavenging rate of DPPH free radicals reached 58.63%±0.45% when its concentration was 14 μg/mL, and the scavenging rate of hydroxyl free radicals reached 52.51%±1.49% when its concentration was 0.08 mg/mL. The experimental results of lipid-lowering activity showed that the inhibitory rate of Phellinus igniarius total flavonoids on pancreatic lipase reached 10.56%±0.06% at the concentration of 6 mg/mL, and the inhibitory rate on cholesterol micelle solubility reached 32.59%±0.78% at the concentration of 4 mg/mL. Phellinus igniarius total flavonids had obvious hypoglycemic activity, and its IC₅₀ for α-glucosidase and α-amylase was 71.42 mg/mL and 97.28 mg/mL respectively.
- Phellinus igniarius,
- total foavonoids,
- extraction,
- component analysis,
- biological activity

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Science and Technology of Food Industry

Extraction, Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius

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