Analysis of Microbial Diversity and Flavor Quality of Chopped Pepper Radish in Post-ripening

AN Lianxia; SU Wei; MU Yingchun; YIN Xuedong

doi:10.13386/j.issn1002-0306.2022120232

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 128-139. > DOI: 10.13386/j.issn1002-0306.2022120232

AN Lianxia, SU Wei, MU Yingchun, et al. Analysis of Microbial Diversity and Flavor Quality of Chopped Pepper Radish in Post-ripening[J]. Science and Technology of Food Industry, 2023, 44(19): 128−139. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120232.

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Analysis of Microbial Diversity and Flavor Quality of Chopped Pepper Radish in Post-ripening

AN Lianxia^{1, 2,},
SU Wei^{1, 2, ,},
MU Yingchun¹,
YIN Xuedong³

1.
School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
2.
Key Laboratory of Storage and Processing Technology Agricultural and Livestock Products, Guizhou University, Guiyang 550025, China
3.
Guisanhong Food Co., Ltd., Zunyi 563099, China

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Received Date: December 29, 2022
Available Online: August 04, 2023

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Abstract

Abstract

Comprehending the changes in the microbial community and volatile substances throughout the post-ripening stage of chopped pepper radish is particularly important for revealing the formation of the distinctive flavor of fermented peppers. The microbial communities and volatile compounds of chopped pepper radish in the post-ripening stage were examined in this work using high-throughput sequencing (HTS) and headspace solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), as well as their underlying correlations. Results indicated that 11 dominant fungal genera and 21 dominant bacterial genera (average relative abundance more than 1%) were filtered out. Sensory analysis showed that the flavor and taste of chopped pepper radish were significantly improved after post-ripening (P<0.05). A total of 74 volatile flavor compounds, mainly alcohols and esters, were detected in chopped pepper radish. According to correlation analysis, 29 volatile flavor substances and 11 core microbial genera (|cor|>0.7 and P<0.05) were significantly related to one another. Pediococcus, Lactococcus, and Penicillium were significantly related to 11, 6, and 5 flavor substances, respectively. The findings in this study demonstrated how metabolites changed as chopped pepper radish underwent the post-ripening process and offered evidence to support efforts to raise the quality of chopped pepper radish.
- chopped pepper radish,
- high-throughput sequencing (HTS),
- microorganisms,
- volatile flavor compounds,
- correlation analysis

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References

[1]	ALVAREZ-PARRILLA E, DE LA ROSA L A, AMAROWICZ R, et al. Antioxidant activity of fresh and processed jalapeno and serrano peppers[J]. Journal of Agricultural and Food Chemistry,2011,59(1):163−173. doi: 10.1021/jf103434u
[2]	ZHUANG Y L, CHEN L, SUN L P, et al. Bioactive characteristics and antioxidant activities of nine peppers[J]. Journal of Functional Foods,2012,4(1):331−338. doi: 10.1016/j.jff.2012.01.001
[3]	李旭阳, 何余堂, 解玉梅, 等. 剁椒扇贝酱工艺研究及贮藏品质分析[J]. 中国食品学报,2022,22(4):225−232. [LI X Y, HE Y T, XIE Y M, et al. Studies on technology and analysis on the storage quality of scallop sauce with chopped pepper[J]. Journal of Chinese Institute of Food Science and Technology,2022,22(4):225−232. LI X Y, HE Y T, JIE Y M, et al. Studies on technology and analysis on the storage quality of scallop sauce with chopped pepper [J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(4): 225-232.
[4]	蔡达, 江育荧, 王文正, 等. 山东、四川不同类型萝卜营养品质分析与综合评价[J]. 食品安全质量检测学报,2022,13(8):2649−2657. [CAI D, JIANG Y Y, WANG W Z, et al. Analysis and comprehensive evaluation of nutritional quality of different types of radishes in Shandong and Sichuan Province[J]. Journal of Food Safety & Quality,2022,13(8):2649−2657. CAI D, JIANG Y Y, WANG W Z, et al. Analysis and comprehensive evaluation of nutritional quality of different types of radishes in Shandong and Sichuan Province [J]. Journal of Food Safety & Quality, 2022, 13(8): 2649-2657.
[5]	GUTIERREZ R M P, PEREZ R L. Raphanus sativus (radish): Their chemistry and biology[J]. The Scientific World Journal,2004,4:873172.
[6]	XU X X, WU B B, ZHAO W T, et al. Shifts in autochthonous microbial diversity and volatile metabolites during the fermentation of chili pepper (Capsicum frutescens L.)[J]. Food Chemistry,2021,335:127512. doi: 10.1016/j.foodchem.2020.127512
[7]	DENG W, WANG M, LI Z, et al. Effect of the changs of microbial community on flavor components of traditional soybean paste during storage period[J]. Food Research International,2022,161:111866. doi: 10.1016/j.foodres.2022.111866
[8]	MA D, LI Y, CHEN C, et al. Microbial succession and its correlation with the dynamics of volatile compounds involved in fermented minced peppers[J]. Frontiers in Nutrition,2022,9:1041608. doi: 10.3389/fnut.2022.1041608
[9]	MEILUN L, XINXING X, SHUANG B, et al. Identification and validation of core microbes associated with key aroma formation in fermented pepper paste (Capsicum annuum L.)[J]. Food Research International,2023,163:112194. doi: 10.1016/j.foodres.2022.112194
[10]	罗凤莲, 夏延斌, 欧阳建勋, 等. 剁辣椒发酵过程中滋味物质的变化[J]. 食品科学,2013,34(3):21−24. [LUO F L, XIA Y B, OUYANG J X, et al. Changes in tasty materials during fermentation of chopped chili[J]. Food Science,2013,34(3):21−24. LUO F L, XIA Y B, OUYANG J X, et al. Changes in tasty materials during fermentation of chopped chili [J]. Food Science, 2013, 34(3): 21-24.
[11]	史婷, 高甜甜, 刘伟, 等. 不同发酵剂对剁辣椒品质的影响[J]. 食品与发酵工业,2022,48(15):144−153. [SHI T, GAO T T, LIU W, et al. Effect of different starters on quality of chopped pepper[J]. Food and Fermentation Industries,2022,48(15):144−153. SHI T, GAO T T, LIU W, et al. Effect of different starters on quality of chopped pepper [J]. Food and Fermentation Industries, 2022, 48(15): 144-153.
[12]	郑红, 唐毅, 王森, 等. 剁椒发酵工艺及贮藏过程品质变化研究[J]. 中国调味品,2020,45(1):155−159. [ZHENG H, TANG Y, WANG S, et al. Study on fermentation technology and quality change of chopped pepper during storage[J]. China Condiment,2020,45(1):155−159. ZHENG H, TANG Y, WANG S, et al. Study on fermentation technology and quality change of chopped pepper during storage [J]. China Condiment, 2020, 45(1): 155-159.
[13]	CALLAHAN B J, MCMURDIE P J, ROSEN M J, et al. DADA2: High-resolution sample inference from Illumina amplicon data[J]. Nature Methods,2016,13(7):581−587. doi: 10.1038/nmeth.3869
[14]	BOKULICH N A, KAEHLER B D, RIDEOUT J R, et al. Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin[J]. Microbiome,2018,6(1):90−107. doi: 10.1186/s40168-018-0470-z
[15]	王景. 16S rRNA基因二代测序中的测序深度与测序错误对微生物群落多样性分析的影响[D]. 上海: 上海交通大学, 2018. I−II. WANG J. The effects of sequencing depth and sequencing errors in the second generation sequencing of 16S rRNA gene on the analysis of microbial community diversity[D]. Shanghai: Shanghai Jiaotong University, 2018. I−II.
[16]	WANG J, WANG R, XIAO Q, et al. Analysis of bacterial diversity during fermentation of Chinese traditional fermented chopped pepper[J]. Letters in Applied Microbiology,2019,69(5):346−352. doi: 10.1111/lam.13212
[17]	MENGJUAN C, YEYOU Q, FANGMING D, et al. Illumina MiSeq sequencing reveals microbial community succession in salted peppers with different salinity during preservation[J]. Food Research International,2021,143:110234. doi: 10.1016/j.foodres.2021.110234
[18]	WILLIAM H U, BOLTON B M. A manual of bacteriology[J]. The American Journal of the Medical Sciences,1910,139(3):436−436. doi: 10.1097/00000441-191003000-00024
[19]	SALZER W, SEIBEL N, SMITH M. Erwinia asparaginase in pediatric acute lymphoblastic leukemia[J]. Expert Opinion on Biological Therapy,2012,12(10):1407−1414. doi: 10.1517/14712598.2012.718327
[20]	RAU R E, DREYER Z, CHOI M R, et al. Outcome of pediatric patients with acute lymphoblastic leukemia/lymphoblastic lymphoma with hypersensitivity to pegaspargase treated with PEGylated Erwinia asparaginase, pegcrisantaspase: A report from the Children's Oncology Group[J]. Pediatr Blood Cancer,2018,65(3):e26873. doi: 10.1002/pbc.26873
[21]	RAO Y, QIAN Y, TAO Y F, et al. Characterization of the microbial communities and their correlations with chemical profiles in assorted vegetable Sichuan pickles[J]. Food Control,2020,113:107174. doi: 10.1016/j.foodcont.2020.107174
[22]	陈添艳, 苏伟, 母应春, 等. 剁椒山笋后熟过程中微生物与挥发性风味物质的相关系研究[J]. 食品科学,2022,44(6):180−189. [CHEN T Y, SU W, MU Y C, et al. Study on the relationship between microorganism and volatile flavor substances during post-ripening process of chopped pepper bamboo shoots[J]. Food Science,2022,44(6):180−189. CHEN T Y, SU W, MU Y C, et al. Study on the relationship between microorganism and volatile flavor substances during post-ripening process of chopped pepper bamboo shoots [J]. Food Science, 2022, 44(6): 180-189.
[23]	SHIVAS R G, TAN Y P, EDWARDS J, et al. Colletotrichum species in Australia[J]. Australasian Plant Pathology,2016,45(5):447−464. doi: 10.1007/s13313-016-0443-2
[24]	WU J R, TIAN T, LIU Y M, et al. The dynamic changes of chemical components and microbiota during the natural fermentation process in Da-Jiang, a Chinese popular traditional fermented condiment[J]. Food Research International,2018,112:457−467. doi: 10.1016/j.foodres.2018.06.021
[25]	MU Y, SU W, YU X T, et al. Untargeted metabolomics based on GC-TOF-MS reveals the optimal pre-fermentation time for black glutinous rice wine[J]. International Journal of Food Properties,2019,22(1):2033−2046. doi: 10.1080/10942912.2019.1705481
[26]	杨洋, 杨宇航, 王洪伟, 等. 不同发酵剂对泡萝卜挥发性风味成分及感官品质的影响[J]. 食品与发酵工业,2023,49(4):175−184. [YANG Y, YANG Y H, WANG H W, et al. Effects of different starter on volatile flavor components and sensory properties of pickled radish[J]. Food and Fermentation Industries,2023,49(4):175−184. YANG Y, YANG Y H, WANG H W, et al. Effects of different starter on volatile flavor components and sensory properties of pickled radish [J]. Food and Fermentation Industries, 2023, 49(4): 175-184.
[27]	ZAREI N, GOLMAKANI M T, KERAMAT M, et al. Process intensification for the autocatalytic esterification of citronellol using microwave radiation[J]. LWT-Food Science and Technology,2021,145:111358. doi: 10.1016/j.lwt.2021.111358
[28]	MCGINTY D, SCOGNAMIGLIO J, LETIZIA C S, et al. Fragrance material review on 2-ethyl-1-hexanol[J]. Food and Chemical Toxicology,2010,48:S115−S29. doi: 10.1016/j.fct.2010.05.042
[29]	WANG J J, WANG R R, XIAO Q, et al. SPME/GC-MS characterization of volatile compounds of Chinese traditional-chopped pepper during fermentation[J]. International Journal of Food Properties,2019,22(1):1863−1872. doi: 10.1080/10942912.2019.1684320
[30]	孙宝国. 食用调香技术[M]. 北京: 化学工业出版社, 2015: 10−28. SUN B G. Edible flavoring technology[M]. Beijing: Chemical Industry Press, 2015: 10−28.
[31]	GOGUS F, OZEL M Z, KESKIN H, et al. Volatiles of fresh and commercial sweet red pepper pastes: Processing methods and microwave assisted extraction[J]. International Journal of Food Properties,2015,18(8):1625−1634. doi: 10.1080/10942912.2014.923910
[32]	LEE S M, LEE J Y, CHO Y J, et al. Determination of volatiles and carotenoid degradation compounds in red pepper fermented by Lactobacillus parabuchneri[J]. Journal of Food Science,2018,83(8):2083−2091. doi: 10.1111/1750-3841.14221
[33]	石华治, 王娟, 刘玉平, 等. 剁椒关键性香气成分分析[J]. 食品安全质量检测学报,2018,9(15):3976−3983. [SHI H Z, WANG J, LIU Y P, et al. Identification of the key odorants in chili pepper[J]. Journal of Food Safety & Quality,2018,9(15):3976−3983. SHI H Z, WANG J, LIU Y P, et al. Identification of the key odorants in chili pepper [J]. Journal of Food Safety & Quality, 2018, 9(15): 3976-3983.
[34]	MAICAS S, MATEO J J. Hydrolysis of terpenyl glycosides in grape juice and other fruit juices: A review[J]. Applied Microbiology and Biotechnology,2005,67(3):322−335. doi: 10.1007/s00253-004-1806-0

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Analysis of Microbial Diversity and Flavor Quality of Chopped Pepper Radish in Post-ripening

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