Simultaneous Determination of 76 Veterinary Drugs and Their Metabolites in Pork by QuEChERS-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

WU Yanlei; SU Min; ZHOU Chunjie; TIAN Yuan; WANG Min

doi:10.13386/j.issn1002-0306.2022110330

Volume 44 Issue 20

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(20): 311-321. > DOI: 10.13386/j.issn1002-0306.2022110330

WU Yanlei, SU Min, ZHOU Chunjie, et al. Simultaneous Determination of 76 Veterinary Drugs and Their Metabolites in Pork by QuEChERS-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(20): 311−321. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110330.

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Simultaneous Determination of 76 Veterinary Drugs and Their Metabolites in Pork by QuEChERS-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

Chongqing Institute for Food and Drug Control, Key Laboratory of Condiment Supervision Technology forState Market Regulation, Chongqing 401121, China

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Received Date: November 30, 2022
Available Online: August 15, 2023

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Abstract

In order to improve the efficiency of the detection of multiple veterinary drug residues in pork, a method for the simultaneous determination of 5 types of 76 veterinary drugs and their metabolites residues in pork, including quinolones, sulfonamides, nitroimidazoles, macrolides and benzimidazoles, was established by QuEChERS combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted with acidified acetonitrile and salted out by anhydrous sodium sulfate. The extract was divided into two parts after purified by C₁₈ and anhydrous magnesium sulfate. One part was used for direct determination of the 22 weakly polar compounds, and the other part was concentrated by nitrogen blowing for the determination of the 54 strongly polar compounds. The 76 compounds were analyzed in multiple reaction monitoring (MRM) mode via electrospray ionization, and quantified by matrix matching external standard method. The results showed that the correlation coefficients were greater than 0.995, and the limits of quantification (LOQs) were lower than 10 μg/kg. The recoveries ranged from 61.3% to 96.2%, with the relative standard deviation (RSD) between 2.0% and 14.6%. The method was applied to 170 pork samples sold in the market, 23 samples of which were detected with 2 types of 5 veterinary drugs residues. The method was simple, rapid, accurate, reliable, and suitable for rapid screening and accurate detection of multiple veterinary drug residues in pork.

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Science and Technology of Food Industry

Simultaneous Determination of 76 Veterinary Drugs and Their Metabolites in Pork by QuEChERS-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

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