Extraction, Purification, Structural Characterization and Antioxidant Activity of Polysaccharides from the Fruiting Body of Guanxian <i>Ganoderma lucidum</i>

LIU Yangshan; ZHANG Jing; REN Hongfei; PANG Mengyu; SUI Xinyi; DU Xiuju

doi:10.13386/j.issn1002-0306.2022080313

Volume 44 Issue 12

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(12): 81-89. > DOI: 10.13386/j.issn1002-0306.2022080313

LIU Yangshan, ZHANG Jing, REN Hongfei, et al. Extraction, Purification, Structural Characterization and Antioxidant Activity of Polysaccharides from the Fruiting Body of Guanxian Ganoderma lucidum[J]. Science and Technology of Food Industry, 2023, 44(12): 81−89. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080313.

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Extraction, Purification, Structural Characterization and Antioxidant Activity of Polysaccharides from the Fruiting Body of Guanxian Ganoderma lucidum

College of Life Sciences, Liaocheng University, Liaocheng 252000, China

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Received Date: August 30, 2022
Available Online: April 18, 2023

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Abstract

Taking Ganoderma lucidum in Guanxian County as the experimental material, the crude polysaccharide GLP was extracted from the fruiting body of G. lucidum by hot water. After deproteinization by Sevag methods and decolorization by activated carbon methods, and then separation and purification by Capto^TM DEAE ion chromatography and Superdex 6 prep Grad gel chromatography, respectively, the homogeneous polysaccharide GLPS80a was obtained. And its average molecular weight was 9024 Da. Its chemical structure was preliminarly characterized by monosaccharide composition analysis, infrared scanning and nuclear magnetic resonance analysis, respectively. The results showed that GLPS80a was composed of fucose, glucosamine, galactose, glucose, xylose, mannose and gluconal, and the molar percentage was 0.06:0.23:0.17:1.00:0.08:0.19:0.23. It was an acid polysaccharide with the main of pyranoid glucose linked by β-D-(1,6) glycosidic bond and did not have a triple helix structure. The antioxidant results indicated that GLPS80a had scavenging ability of DPPH radical, ·OH radical and ABTS⁺ radical. Their EC₅₀ was 7.40, 8.74 and 0.33 mg/mL, respectively, and the reducing power RP_0.5AU was 8.33 mg/mL. This work would lay a theoretical foundation for development of G. lucidum resources in Guanxian County.
- Ganoderma lucidum,
- polysaccharides,
- separation and purification,
- structural characterization,
- antioxidant activity

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Science and Technology of Food Industry

Extraction, Purification, Structural Characterization and Antioxidant Activity of Polysaccharides from the Fruiting Body of Guanxian Ganoderma lucidum

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