Separation, Purification and Antioxidant Activity of Selenium Polysaccharide from Selenium-enriched Grapes

ZHANG Jinrong; LU Shiling; LUO Ruifeng; MA Xiaoning; WANG Guodong

doi:10.13386/j.issn1002-0306.2022070170

Volume 44 Issue 8

Apr. 2023

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Science and Technology of Food Industry > 2023 > 44(8): 116-124. > DOI: 10.13386/j.issn1002-0306.2022070170

ZHANG Jinrong, LU Shiling, LUO Ruifeng, et al. Separation, Purification and Antioxidant Activity of Selenium Polysaccharide from Selenium-enriched Grapes[J]. Science and Technology of Food Industry, 2023, 44(8): 116−124. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070170.

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Separation, Purification and Antioxidant Activity of Selenium Polysaccharide from Selenium-enriched Grapes

1.
College of Food Science, Shihezi University, Shihezi 832000, China
2.
Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China

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Received Date: July 18, 2022
Available Online: February 15, 2023

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Abstract

In this paper, selenium polysaccharide (sKGP) extract was obtained by extraction, separation and purification of selenium-enriched creeson grape, then its physicalchemical properties, structural characteristics and antioxidant activity were studied. The selenium polysaccharide was extracted by ultrasonic-assisted enzymatic, separated and purified by diethylaminoethyl cross-linked dextran gel chromatography column. Its active functional groups and structures were identified by UV, LC-MS, fourier IR and SEM, and the antioxidant activity indexes were also analyzed. The results showed that the content of selenium in sKGP-3 was (0.642±0.038) mg/kg, and the purified sKGP-3 contained almost no nucleic acid and protein, the sKGP-3 was composed of rhamnose, xylose, arabinose, mannose, glucose and fructose, the molar ratio was 0.172:0.743:0.718:0.082:4.540:0.093. The sKGP-3 had the structure of Se=O, Se-H and typical characteristic peaks of polysaccharides, and the shape of the sKGP-3 was mainly lamellar and filamentous, belonging to amorphous state. The evaluation of antioxidant activity in vitro showed that the sKGP had higher antioxidant activity than the polysaccharide (KGP) of the same concentration before purification, the total antioxidant capacity of sKGP was 8.499 μmol/mL, and the scavenging capacity of DPPH, hydroxyl radicals and ABTS⁺ radicals were 69.7%, 85.8% and 92.5%, respectively.
- grape selenium polysaccharide,
- separation and purification,
- structure identification,
- antioxidant activity

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[1]	GUO C H, HSIA S, CHEN P C. Distribution of selenium and oxidative stress in breast tumor-bearing mice[J]. Nutrients,2013,5(2):594−607. doi: 10.3390/nu5020594
[2]	WANG L, LI X Y, WANG B B. Synthesis, characterization and antioxidant activity of selenium modified polysaccharides from Hohenbuehelia serotina[J]. International Journal of Biological Macromolecules,2018,120:1362−1368. doi: 10.1016/j.ijbiomac.2018.09.139
[3]	VINCETI M, CRESPI C M, BONVICINI F, et al. The need for a reassessment of the safe upper limit of selenium in drinking water[J]. Science of the Total Environment,2013,443(15):633−642.
[4]	GAO Z, ZHANG C, LIU H, et al. The characteristics and antioxidation of Oudemansiella radicata selenium polysaccharides on lipopolysaccharide-induced endo-toxemic mice[J]. International Journal of Biological Macromolecules,2018,116:753−764. doi: 10.1016/j.ijbiomac.2018.05.078
[5]	LI Q, WANG W, ZHU Y, et al. Structural elucidation and antioxidant activity a novel Se-polysaccharide from Se-enriched Grifola frondosa[J]. Carbohydrate Polymers,2017,161:42−52. doi: 10.1016/j.carbpol.2016.12.041
[6]	WANG L B, LI L Y, GAO J Y, et al. Characterization, antioxidant and immunomodulatory effects of selenized polysaccharides from dandelion roots[J]. Carbohydrate Polymers,2021,260:117796. doi: 10.1016/j.carbpol.2021.117796
[7]	ZHU Z Y, LIU F, GAO H, et al. Synthesis, characterization and antioxidant activity of selenium polysaccharide from Cordyceps militaris[J]. International Journal of Biological Macromolecules,2016,93:1090−1099. doi: 10.1016/j.ijbiomac.2016.09.076
[8]	MA L, LIU J, LIU A J, et al. Cytoprotective effect of Selenium polysaccharide from Pleurotus ostreatus against H₂O₂-induced oxidative stress and apoptosis in PC12 cells[J]. Arabian Journal of Chemistry,2022,15(4):103686. doi: 10.1016/j.arabjc.2022.103686
[9]	LI J, SHEN B X, NIE S L, et al. A combination of selenium and polysaccharides: Promising therapeutic potential[J]. Carbohydrate Polymers,2018,206:163−173.
[10]	OGRA Y, SHIMIZU M, TAKAHASHI K, et al. Biotransformation of organic selenium compounds in budding yeast, Saccharomyces cerevisiae[J]. Metallomics,2018,10(9):1257−1263. doi: 10.1039/C8MT00176F
[11]	CHENG L Z, WANG Y F, HE X X, et al. Preparation, structural characterization and bioactivities of Se-containing polysaccharide: A review[J]. International Journal of Biological Macromolecules,2018,120:82−92. doi: 10.1016/j.ijbiomac.2018.07.106
[12]	LIU Y, HUANG G L. Extraction and derivatisation of active polysaccharides[J]. Journal of Enzyme Inhibition Medicinal Chemistry,2019,34(1):1690−1696. doi: 10.1080/14756366.2019.1660654
[13]	钟海霞, 潘明启, 张付春, 等. 不同砧木对克瑞森葡萄果实可溶性糖含量的影响[J]. 新疆农业科学,2018,55(9):1633−1638. [ZHONG H X, PAN M Q, ZHANG F C, et al. Effect of different rootstocks on soluble sugar content of cressen grape fruit[J]. Xinjiang Agricultural Science,2018,55(9):1633−1638.
[14]	LIU Y, SUN Y Y, HUANG G L. Preparation and antioxidant activities of important traditional plant polysaccharides[J]. International Journal of Biological Macromolecules,2018,111:780−786. doi: 10.1016/j.ijbiomac.2018.01.086
[15]	HUANG S Y, YANG W J, HUANG G L. Preparation and activities of selenium polysaccharide from plant such as Grifola frondosa[J]. Carbohydrate Polymers,2020,242:116409. doi: 10.1016/j.carbpol.2020.116409
[16]	GU Y G, QIU Y, WEI X, et al. Characterization of selenium-containing polysaccharides isolated from selenium-enriched tea and its bioactivities[J]. Food Chemistry,2020,316:126371. doi: 10.1016/j.foodchem.2020.126371
[17]	中华人民共和国国家卫生健康委员会, 国家市场监督管理局. GB 5009.93-2017[S]. 北京: 中国标准出版社, 2017: 1−3 National Health Commission of the People's Republic of China, State Administration of Market Regulation. GB 5009.93-2017[S]. Beijing: China Standards Press, 2017: 1−3.
[18]	郭慧静. 蒲公英多糖的提取, 分离纯化, 鉴定及其生物活性的初步研究[D]. 石河子: 石河子大学, 2019 GUO H J. A preliminary study on the extraction, separation, purification, identification and biological activity of dandelion polysaccharide[D]. Shihezi: Shihezi University, 2019.
[19]	CHENG H, HUANG G L. Extraction, characterisation and antioxidant activity of Allium sativum polysaccharide[J]. International Journal of Biological Macromolecules,2018,114:415−419. doi: 10.1016/j.ijbiomac.2018.03.156
[20]	王川丕, 诸力, 刘新, 等. 超高效液相色谱-质谱联用技术测定茶叶中8种单, 寡糖的含量[J]. 食品科学,2014,20:6. [WANG C P, ZHU L, LIU X, et al. Determination of 8 kinds of monosaccharides and oligosaccharides in tea by ultra high performance liquid chromatography-mass spectrometry[J]. Food Science,2014,20:6.
[21]	GAO Z, ZHANG C, TIAN C Y, et al. Characterization, antioxidation, anti-inflammation and renoprotection effects of selenized mycelia polysaccharides from Oudemansiella radicata[J]. Carbohydrate Polymers,2018,181:1224−1234. doi: 10.1016/j.carbpol.2017.12.007
[22]	CHEN G J, KAN J Q. Characterization of a novel polysaccharide isolated from Rosa roxburghii Tratt fruit and assessment of its antioxidant in vitro and in vivo[J]. International Journal of Biological Macromolecules,2018,107:166−174. doi: 10.1016/j.ijbiomac.2017.08.160
[23]	ZHU J X, CHEN Z Y, CHEN L, et al. Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea[J]. International Journal of Biological Macromolecules,2019,130:388−398. doi: 10.1016/j.ijbiomac.2019.02.102
[24]	TENG C, QIN P Y, SHI Z X, et al. Structural characterization and antioxidant activity of alkali-extracted polysaccharides from quinoa[J]. Food Hydrocolloids,2020,113:106392.
[25]	XIE L M, SHEN M Y, WEN P W, et al. Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus[J]. Food and Chemical Toxicology,2021,145:111754.
[26]	WU C Z, ZHAO M M, BU X Y, et al. Preparation, characterization, antioxidant and antiglycation activities of selenized polysaccharides from blackcurrant[J]. RSC Advances,2020,10(54):32616−32627. doi: 10.1039/D0RA06462A
[27]	LAPUENTE L M, GUADALUPE Z, AYESTARAN B, et al. Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition[J]. Food Chemistry,2021,356(10):129669.
[28]	LIU G Y, YANG X, ZHANG J X, et al. Synthesis, stability and anti-fatigue activity of selenium nanoparticles stabilized by Lycium barbarum polysaccharides[J]. International Journal of Biological Macromolecules,2021,179:418−428. doi: 10.1016/j.ijbiomac.2021.03.018
[29]	LUO L J, WANG Y X, ZHANG S, et al. Preparation and characterization of selenium-rich polysaccharide from Phellinus igniarius and its effects on wound healing[J]. Carbohydrate Polymers,2021,264(6):117982.
[30]	HOU R R, CHEN J, YUE C J, et al. Modification of lily polysaccharide by selenylation and the immune-enhancing activity[J]. Carbohydrate Polymers,2016,142:73−81. doi: 10.1016/j.carbpol.2016.01.032
[31]	张军耀. 富硒黑茶多糖的提取分离纯化及性质研究[D]. 上海: 上海师范大学, 2019 ZHANG J Y. Extraction, purification and properties of selenium enriched black tea polysaccharide[D]. Shanghai: Shanghai Normal University, 2019.
[32]	LIU X G, LIU J, LIU C F, et al. Selenium-containing polysaccharides isolated from Rosa laevigata Michx fruits exhibit excellent anti-oxidant and neuroprotective activity in vitro[J]. International Journal of Biological Macromolecules,2022,209(PtA):1222−1233.
[33]	DONG F, ZHENG H Z, JEONG W S, et al. Synthesis, characterization, and antioxidant activity in vitro of selenium-Euryale ferox Salisb. polysaccharide[J]. Applied Biological Chemistry,2021,64(1):59. doi: 10.1186/s13765-021-00631-4
[34]	DONG Z, DONG G, LAI F R, et al. Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma lucidum mycelia[J]. International Journal of Biological Macromolecules,2021,190(6):101−112.
[35]	MA L, ZHAO Y N, YU J, et al. Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro[J]. International Journal of Biological Macromolecules,2018,111:421−429. doi: 10.1016/j.ijbiomac.2017.12.152

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Science and Technology of Food Industry

Separation, Purification and Antioxidant Activity of Selenium Polysaccharide from Selenium-enriched Grapes

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