Lactic Acid Fermentation by <i>Bacillus coagulans</i> with Mixed Carbon Sources

CHEN Minghao; LIU Zhihao; WANG Yonghong

doi:10.13386/j.issn1002-0306.2022050186

Volume 44 Issue 6

Mar. 2023

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Science and Technology of Food Industry > 2023 > 44(6): 155-161. > DOI: 10.13386/j.issn1002-0306.2022050186

CHEN Minghao, LIU Zhihao, WANG Yonghong. Lactic Acid Fermentation by Bacillus coagulans with Mixed Carbon Sources[J]. Science and Technology of Food Industry, 2023, 44(6): 155−161. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050186.

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Lactic Acid Fermentation by Bacillus coagulans with Mixed Carbon Sources

School of Biotechnology, East China University of Science and Technology, Shanghai 200000, China

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Received Date: May 16, 2022
Available Online: January 10, 2023

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Abstract

Abstract

To better understand the metabolism in industrial lactic acid fermentation by Bacillus coagulans, the lactic acid fermentation characteristics with mixed carbon sources were studied under varied aeration, pH, and neutralizer conditions in laboratory-scale bioreactor, and the effects of metal ions and osmotic pressure on the autolysis of B. coagulans under starvation were conducted. The results showed that the lactic acid fermentation by B. coagulans using glucose+trehalose as the mixed carbon source showed a significant carbon catabolite repression (CCR), and the fermentation process could be divided into glucose consumption phase (phase I), organic acids consumption phase (phase II) and trehalose consumption phase (phase III). Differents fermentation conditions had less of an impact on cell growth and lactic acid production at phase I, but had a considerable impact on phase III. In the phase III, the highest trehalose consumption rate and lactic acid productivity appeared at 7.2 L/h (0.03 vvm) ventilation. The rates of sugar consumption and lactic acid production were higher at pH6.5 than that at pH6.0, while nearly no trehalose consumption and lactic acid production were observed at pH5.5. Compared with NaOH, when Ca(OH)₂ was used as a neutralizer at pH6.5, the trehalose utilization rate and lactic acid production rate were enhanced by 21.0% and 28.3%, respectively. It was found that Ca²⁺ and Mg²⁺ alleviated autolysis, while Na⁺ had some benefits in maintaining the activity of cells. The results of this paper would lay the foundation for studying the mechanism of metabolic shift during lactic acid fermentation by B. coagulans in mixed carbon sources.
- Bacillus coagulans,
- mixed carbon sources,
- fermentation characteristics,
- metal ions,
- autolysis

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[1]	WANG C, CUI Y, QU X. Mechanisms and improvement of acid resistance in lactic acid bacteria[J]. Archives of Microbiology,2018,200(2):195−201. doi: 10.1007/s00203-017-1446-2
[2]	HOU Y, GAO B, CUI J, et al. Combination of multi-enzyme expression fine-tuning and co-substrates addition improves phenyllactic acid production with an Escherichia coli whole-cell biocatalyst[J]. Bioresource Technology,2019,287:121423. doi: 10.1016/j.biortech.2019.121423
[3]	赵士友, 吴润光, 杨婷婷, 等. 立构复合聚乳酸纤维制备技术的研究进展[J]. 棉纺织技术,2021,49(11):79−84. [ZHAO Shiyou, WU Runguang, YANG Tingting, et al. Research progress of stereo-complexed polylactide fiber preparation technology[J]. Cotton Textile Technology,2021,49(11):79−84. doi: 10.3969/j.issn.1001-7415.2021.11.022
[4]	WANG Y, CAO W, LUO J, et al. One step open fermentation for lactic acid production from inedible starchy biomass by thermophilic Bacillus coagulans IPE22[J]. Bioresource Technology,2019,272:398−406. doi: 10.1016/j.biortech.2018.10.043
[5]	BU C Y, YAN Y X, ZOU L H, et al. One-pot biosynthesis of furfuryl alcohol and lactic acid via a glucose coupled biphasic system using single Bacillus coagulans NL01[J]. Bioresource Technology,2020,313:123705. doi: 10.1016/j.biortech.2020.123705
[6]	ALVES DE OLIVEIRA R, SCHNEIDER R, VAZ ROSSELL C E, et al. Polymer grade l-lactic acid production from sugarcane bagasse hemicellulosic hydrolysate using Bacillus coagulans[J]. Bioresource Technology Reports,2019,6:26−31. doi: 10.1016/j.biteb.2019.02.003
[7]	孙研, 王永红. 不同凝结芽孢杆菌在单一及混合碳源下的发酵特性[J]. 食品工业科技,2020,41(16):74−80. [SUN Yan, WANG Yonghong. Fermentation characteristics of different strains of Bacillus coagulans with single and mixed carbon sources[J]. Science and Technology of Food Industry,2020,41(16):74−80. doi: 10.13386/j.issn1002-0306.2020.16.013
[8]	NAIR A, SARMA S J. The impact of carbon and nitrogen catabolite repression in microorganisms[J]. Microbiological Research,2021,251:126831. doi: 10.1016/j.micres.2021.126831
[9]	ABDEL RAHMAN M A, HASSAN S E D, ALREFAEY H M A, et al. Efficient co-utilization of biomass-derived mixed sugars for lactic acid production by Bacillus coagulans Azu-10[J]. Fermentation,2021,7(1):28−45. doi: 10.3390/fermentation7010028
[10]	ZIMMERMAN T, IBRAHIM S A. Autolysis and cell death is affected by pH in L. reuteri DSM 20016 cells[J]. Foods,2021,10(1026):1026.
[11]	ABE K, TOYOFUKU M, NOMURA N, et al. Autolysis-mediated membrane vesicle formation in Bacillus subtilis[J]. Environmental Microbiology,2021,23(5):2632−2647. doi: 10.1111/1462-2920.15502
[12]	JUTURU V, WU J C. Production of high concentration of l-lactic acid from oil palm empty fruit bunch by thermophilic Bacillus coagulans JI12: Microbial production of l-lactic acid from Lignocellulose[J]. Biotechnology and Applied Biochemistry,2018,65(2):145−149. doi: 10.1002/bab.1567
[13]	CHEN Y, DONG F, WANG Y. Systematic development and optimization of chemically defined medium supporting high cell density growth of Bacillus coagulans[J]. Applied Microbiology and Biotechnology,2016,100(18):8121−8134. doi: 10.1007/s00253-016-7644-z
[14]	孙研. 凝结芽孢杆菌恒化培养及其在混合碳源中的代谢特性研究[D]. 上海: 华东理工大学, 2020 SUN Yan. Study on metabolic characteristics of Bacillus coagulans in chemostat culture and mixed carbon sources[D]. Shanghai: East China University of Science and Technology, 2020.
[15]	VERMASSEN A, LEROY S, TALON R, et al. Cell wall hydrolases in bacteria: insight on the diversity of cell wall amidases, glycosidases and peptidases toward peptidoglycan[J]. Frontiers in Microbiology,2019,10:331. doi: 10.3389/fmicb.2019.00331
[16]	WANG Y, CAO W, LUO J, et al. Exploring the potential of lactic acid production from lignocellulosic hydrolysates with various ratios of hexose versus pentose by Bacillus coagulans IPE22[J]. Bioresource Technology,2018,261:342−349. doi: 10.1016/j.biortech.2018.03.135
[17]	LI F, WEI X, SUN Q, et al. Production of L-lactic acid in Saccharomyces cerevisiae through metabolic engineering and rational cofactor Engineering[J]. Sugar Tech,2022:1−12.
[18]	QU C, CHEN L, LI Y, et al. The redox-sensing transcriptional repressor Rex is important for regulating the products distribution in Thermoanaerobacterium aotearoense SCUT27[J]. Applied Microbiology and Biotechnology,2020,104(12):5605−5617. doi: 10.1007/s00253-020-10554-7
[19]	PARK J, CHOI Y. Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review[J]. Frontiers of Chemical Science and Engineering,2017,11(1):66−71. doi: 10.1007/s11705-016-1591-1
[20]	MISIOU O, ZOUROU C, KOUTSOUMANIS K. Development and validation of a predictive model for the effect of temperature, pH and water activity on the growth kinetics of Bacillus coagulans in non-refrigerated ready-to-eat food products[J]. Food Research International,2021,149:110705. doi: 10.1016/j.foodres.2021.110705
[21]	LAVRENTEV F V, ASHIKHMINA M S, ULASEVICH S A, et al. Perspectives of Bacillus coagulans MTCC 5856 in the production of fermented dairy products[J]. LWT,2021,148:111623. doi: 10.1016/j.lwt.2021.111623
[22]	BAGKAR P, GUPTA A K, MAITY C. Effect of high pressure processing (HPP) on spore preparation of probiotic Bacillus coagulans LBSC [DSM 17654][J]. International Journal of Food Engineering,2021,17(9):747−753. doi: 10.1515/ijfe-2020-0336
[23]	CHEN Y, SUN Y, LIU Z, et al. Genome-scale modeling for Bacillus coagulans to understand the metabolic characteristics[J]. Biotechnology and Bioengineering,2020,117(11):3545−3558. doi: 10.1002/bit.27488
[24]	ZHANG C, ZHOU C, ASSAVASIRIJINDA N, et al. Non-sterilized fermentation of high optically pure D-lactic acid by a genetically modified thermophilic Bacillus coagulans strain[J]. Microbial Cell Factories,2017,16(1):213. doi: 10.1186/s12934-017-0827-1
[25]	TIAN X, WANG Y, CHU J, et al. L-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination[J]. Bioprocess and Biosystems Engineering,2014,37(9):1917−1923. doi: 10.1007/s00449-014-1166-9
[26]	BURCHAM L R, HILL R A, CAULKINS R C, et al. Streptococcus pneumoniae metal homeostasis alters cellular metabolism[J]. Metallomics, The Royal Society of Chemistry,2020,12(9):1416−1427.
[27]	KONG F, REN H Y, ZHAO L, et al. Semi-continuous lipid production and sedimentation of Scenedesmus sp. by metal ions addition in the anaerobic fermentation effluent[J]. Energy Conversion and Management,2020,203:112216. doi: 10.1016/j.enconman.2019.112216
[28]	WANG X, AN P, GU Z, et al. Mitochondrial metal ion transport in cell metabolism and disease[J]. International Journal of Molecular Sciences,2021,22(14):7525. doi: 10.3390/ijms22147525
[29]	ZHONG W, MENG W, ZHANG T, et al. Effects of metal ions on the reduction of penicillin fermentation residue by thermophilic bacteria[J]. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects,2017,39(18):1942−1947. doi: 10.1080/15567036.2017.1390013
[30]	MAYER C, KLUJ R M, MUEHLECK M, et al. Bacteria’s different ways to recycle their own cell wall[J]. International Journal of Medical Microbiology,2019,309(7):151326. doi: 10.1016/j.ijmm.2019.06.006
[31]	LO S C, YANG C Y, MATHEW D C, et al. Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture[J]. Scientific Reports,2021,11(1):14552. doi: 10.1038/s41598-021-94101-y

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