Screening of an Alcohol Tolerant and High-yield L-lactic Acid Strain and Optimization of Culture Medium

XIAO Minmin; XING Xinyue; LIU Wenguang; MENG Xianghui; WEI Shanshan; ZHANG Tianxiao; WANG Yuhua; LI Xia

doi:10.13386/j.issn1002-0306.2022040147

Volume 44 Issue 8

Apr. 2023

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Science and Technology of Food Industry > 2023 > 44(8): 135-143. > DOI: 10.13386/j.issn1002-0306.2022040147

XIAO Minmin, XING Xinyue, LIU Wenguang, et al. Screening of an Alcohol Tolerant and High-yield L-lactic Acid Strain and Optimization of Culture Medium[J]. Science and Technology of Food Industry, 2023, 44(8): 135−143. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040147.

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Screening of an Alcohol Tolerant and High-yield L-lactic Acid Strain and Optimization of Culture Medium

1.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
2.
Huaxin Testing Technology Co., Ltd., Changchun 130000, China

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Received Date: April 13, 2022
Available Online: February 20, 2023

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Abstract

In order to increase the yield of L-lactic acid and reduce the production cost of L-lactic acid, a strain of Lactobacillus rhamnosus AK-0779 with alcohol tolerance and high yield of L-lactic acid was screened and domesticated. Corn distiller's grains were used as the nitrogen source for the fermentation medium of strain AK-0779 instead of part of yeast powder. On the basis of single factor experiments, three factors and three levels response surface optimization experiments were carried out on the addition of glucose, yeast powder and corn distiller's grains. The results showed that the optimal fermentation medium was glucose 9.80%, corn distiller's grains 0.98%, yeast powder 1.72%, L-lactic acid yield 78.91 g/L and sugar acid conversion rate 80.52%. There was no significant difference in the yield of L-lactic acid compared with yeast powder, which was 82.36 g/L, indicating that corn distiller's grains could effectively replace part of yeast powder as the nitrogen source of fermentation medium and reduce the production cost of L-lactic acid.
- Lactobacillus rhamnosus,
- corn distiller's,
- L-lactic acid,
- optimization of fermentation medium,
- response surface analysis

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Science and Technology of Food Industry

Screening of an Alcohol Tolerant and High-yield L-lactic Acid Strain and Optimization of Culture Medium

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