Antioxidant Activity and Pyrolysis Properties of Lignin Extracted from <i>Camellia oleifera</i> Shell by Deep Eutectic Solvents (DES)

LI Feng; LI Yiqing; MAO Haili; HUANG Dena; ZENG Chenglu

doi:10.13386/j.issn1002-0306.2022040112

Volume 43 Issue 24

Dec. 2022

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Science and Technology of Food Industry > 2022 > 43(24): 261-267. > DOI: 10.13386/j.issn1002-0306.2022040112

LI Feng, LI Yiqing, MAO Haili, et al. Antioxidant Activity and Pyrolysis Properties of Lignin Extracted from Camellia oleifera Shell by Deep Eutectic Solvents (DES)[J]. Science and Technology of Food Industry, 2022, 43(24): 261−267. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040112.

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Antioxidant Activity and Pyrolysis Properties of Lignin Extracted from Camellia oleifera Shell by Deep Eutectic Solvents (DES)

LI Feng^{1, 2,},
LI Yiqing¹,
MAO Haili^{1, 2},
HUANG Dena^{1, 2},
ZENG Chenglu^1, ,

1.
School of Chemistry and Chemical Engineering, Qiannan Normal College for Nationalities, Duyun 558000, China
2.
Qiannan Engingineering Research Center for Environmental Governanen and Contamination Control, Duyun 558000, China

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Received Date: April 11, 2022
Available Online: November 08, 2022

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Abstract

Abstract

In order to obtain lignin with low molecular weight and polydispersity, as well as high antioxidant activity, using Camellia oleifera shell as raw material, four lignin products were isolated from it by alkaline and deep eutectic solvents. The structural and pyrolysis properties of lignin were investigated with ultraviolet spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC) and thermogravimetric analyzer (TGA). Furthermore, the antioxidant activity of the lignin samples was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay. The UV and FTIR analyses showed that the lignin from Camellia oleifera shell was mainly composed of guaiacyl and syringyl. GPC analysis revealed that AL had a relatively higher molecular weight (Mw=41858 g/mol) and broad polydispersity index (PDI=4.53), and the DES-lignin exhibited relatively low relative molecular weight (Mw<11000 g/mol) and narrow polydispersity index (PDI<2). Thermogravimetric analysis showed that the thermal stability of lignin samples was ChCl-OA>AL>ChCl-EG-P>ChCl-GA. Furthermore, the results showed that four lignin products had good antioxidant activity in vitro, and the IC₅₀ values of DPPH free radical scavenging ability was 0.388~1.02 mg/mL. The findings of this study would provide a novel way to fractionate lignin from Camellia oleifera shell, and provide certain reference value for the development of lignin valorization.
- Camellia oleifera shell,
- lignin,
- deep eutectic solvents,
- structural characterization,
- antioxidant activity

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Antioxidant Activity and Pyrolysis Properties of Lignin Extracted from Camellia oleifera Shell by Deep Eutectic Solvents (DES)

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