Study on Antioxidant Activity of <i>Tricholoma matsutake</i> Polysaccharide <i>in Vitro</i> and Its Effect on Mice Damaged by Ethanol Oxidation

SHEN Chenglong; CHEN Yifan; ZENG Yingjie; CHEN Lianhong

doi:10.13386/j.issn1002-0306.2022020248

Volume 43 Issue 23

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(23): 371-377. > DOI: 10.13386/j.issn1002-0306.2022020248

SHEN Chenglong, CHEN Yifan, ZENG Yingjie, et al. Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation[J]. Science and Technology of Food Industry, 2022, 43(23): 371−377. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020248.

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Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation

College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China

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Received Date: February 27, 2022
Available Online: October 08, 2022

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Abstract

Abstract

In order to study the antioxidant activity of Tricholoma matsutake polysaccharides (TMP), its antioxidant capacity in vitro was investigated using superoxide anion radical (O₂^－ · ) scavenging capacity, total reducing capacity and ABTS radical scavenging capacity as indicators; and then the mouse model of ethanol oxidative damage was established, and the antioxidant activity of Tricholoma matsutake polysaccharides in vivo were measured by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in the serum of mice and the activity of catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver. In vitro antioxidant results showed that Tricholoma matsutake polysaccharides had better scavenging ability on superoxide anion free radical and ABTS free radical, and the scavenging rate reached 56.67% and 96.60% when the concentration was 1 mg/mL. However, the total reducing ability of Tricholoma matsutake polysaccharides was not obvious. When the concentration was 1 mg/mL, the absorbance value could reach 0.163. Animal experiment results showed that after four weeks of continuous gastric administration, compared with the model control group, Tricholoma matsutake polysaccharides could significantly reduce ALT, AST and AKP viability in mice serum (P<0.05); it also significantly increased the levels of CAT, SOD and GSH-Px in liver (P<0.05), when the content of MDA was significantly reduced (P<0.05). All these indicators showed a dose-response relationship. The experimental results showed that Tricholoma matsutake polysaccharides had certain antioxidant activity, hoping to provide ideas for the subsequent development and application of Tricholoma matsutake polysaccharides.
- Tricholoma matsutake polysaccharide,
- in vitro antioxidant,
- in vivo antioxidant,
- ethanol oxidation damage

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Science and Technology of Food Industry

Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation

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