Nutritional Evaluation of Walnut Protein and Its Ameliorative Effect on DSS Induced Acute Colitis in Mice

LIU Dandan; ZHU Junsong; ZHANG Xueli; CHEN Min; GUO Yiting; MA Haile

doi:10.13386/j.issn1002-0306.2022010066

Volume 43 Issue 20

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(20): 372-379. > DOI: 10.13386/j.issn1002-0306.2022010066

LIU Dandan, ZHU Junsong, ZHANG Xueli, et al. Nutritional Evaluation of Walnut Protein and Its Ameliorative Effect on DSS Induced Acute Colitis in Mice[J]. Science and Technology of Food Industry, 2022, 43(20): 372−379. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010066.

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Nutritional Evaluation of Walnut Protein and Its Ameliorative Effect on DSS Induced Acute Colitis in Mice

LIU Dandan^1,,
ZHU Junsong¹,
ZHANG Xueli¹,
CHEN Min³,
GUO Yiting^{1, 2},
MA Haile^{1, 2, ,}

1.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
2.
Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China
3.
Laboratory Animal Research Center, Jiangsu University, Zhenjiang 212013, China

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Received Date: January 09, 2022
Available Online: July 28, 2022

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Abstract

Abstract

The nutritional value of walnut protein was evaluated based on the amino acid composition, and the effects of walnut protein and walnut protease hydrolysate on disease activity index score, serum inflammatory factors, myeloperoxidase activity and colon tissue morphology were investigated in mice with acute colitis induced by sodium glucan sulfate (DSS). The results showed that walnut protein had a reasonable amino acid composition. The essential amino acid content (26.98%), the predictive value of protein efficacy ratio (PER, 1.55 and 1.58) and the biological value (BV, 87.96%) were high. After digestion in mice, walnut protein and its hydrolysate could retard the onset of colitis, slow down the weight loss of mice caused by colitis, and significantly improve the clinical symptoms. Serum biochemical indexes showed that both walnut protein and its hydrolysate could extremely significantly (P<0.01) reduce the contents of IL-1β, IL-6, TNF-α and myeloperoxidase (MPO) activity in serum of acute colitis mice. The histopathology of colon tissue showed that both walnut protein and its hydrolysate could significantly improve the morphological characteristics of the colon in colitis mice, alleviate the damage of colon tissue, and reduce the infiltration of inflammatory cells. In conclusion, walnut protein could prevent and retard the occurrence and development of acute colitis after gastrointestinal digestion.
- walnut protein,
- hydrolysate,
- nutritional value,
- colitis,
- anti-inflammatory activity

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