WANG Jiyu, LI Chengwen, XU Yanjing, et al. Optimization of the Preparation of Trans-crocetin from Crocin by Alkaline Hydrolysis via Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(23): 176−183. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030048.
Citation: WANG Jiyu, LI Chengwen, XU Yanjing, et al. Optimization of the Preparation of Trans-crocetin from Crocin by Alkaline Hydrolysis via Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(23): 176−183. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030048.

Optimization of the Preparation of Trans-crocetin from Crocin by Alkaline Hydrolysis via Response Surface Methodology

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  • Received Date: March 04, 2021
  • Available Online: October 08, 2021
  • Objective: To optimize the preparation conditions of trans-crocetin from crocin by alkaline hydrolysis for obtaining trans-crocetin with high purity. Methods: A single factor experiment was used to preliminarily investigate the influence of the hydrolysis reaction time, temperature, and alkali concentration on the yield of trans-crocetin. Then the Box-Behnken design response surface experiment was used to optimize the parameters. Results: The best hydrolysis conditions were determined as follows: the reaction time, temperature and alkali concentration were 60.0 min, 80.0 ℃ and 1.70 mg/mL respectively. Under this condition, crude trans-crocetin with purity of 86.0% was obtained, the yield was 80.09%. After recrystallization, the purity of the crude product was increased to 99.3%. The purified product was determined to be all-trans-crocetin by carbon nuclear magnetic resonance (13C NMR) and hydrogen spectrum (1H NMR) analysis. Conclusion: It is an effective method of alkaline hydrolysis from crocin and recrystallization to prepare trans-crocetin with high purity.
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