ZHU Xiuqing, WANG Yuan, ZHU Ying, et al. Effect of Soybean Phosphatidyl Ethanolamine on the Formation and Properties of Pickering Emulsion Stabilized by Hemp Protein Isolate[J]. Science and Technology of Food Industry, 2021, 42(23): 66−72. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020124.
Citation: ZHU Xiuqing, WANG Yuan, ZHU Ying, et al. Effect of Soybean Phosphatidyl Ethanolamine on the Formation and Properties of Pickering Emulsion Stabilized by Hemp Protein Isolate[J]. Science and Technology of Food Industry, 2021, 42(23): 66−72. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020124.

Effect of Soybean Phosphatidyl Ethanolamine on the Formation and Properties of Pickering Emulsion Stabilized by Hemp Protein Isolate

More Information
  • Received Date: February 19, 2021
  • Available Online: October 08, 2021
  • This paper studied the effect of soybean cephalin on hemp protein isolated Pickering emulsion properties. The emulsifying activity index, the microstructure, droplet size, Zeta potential, the influence of rheological properties and storage stability were measured. It was critical to explore the influence mechanism of hemp protein isolated Pickering. The results showed that the stability of Pickering emulsion was improved after the addition of cephalin. The optimum indexes of the Pickering emulsion were obtained when the amount of cephalin was 30%, with an emulsion activity index of 4.63 m2⁄g and a dispersion index of 0.23. The droplet size was 125 nm, and the Zeta potential was −27.12 mV. The apparent viscosity of the emulsion was small. There was no obvious droplet aggregation and oil leakage phenomenon after 7 days storage, therefore the storage stability was good. This study provided a theoretical basis for improving the stability of hemp protein isolate Pickering emulsion, which could also expand the application field of soybean cephalin.
  • loading
  • [1]
    聂梦琳, 饶川艳, 莫明规, 等. 广西火麻膳食纤维提取及其特性研究[J]. 食品安全质量检测学报,2020,11(17):6187−6195. [NIE Menglin, RAO Chuanyan, MO Minggui, et al. Study on the extraction of Guangxi hemp dietary fiber and its characteristics[J]. Journal of Food Safety & Quality,2020,11(17):6187−6195.
    [2]
    PAULITZ J, SIGMUND I, KOSAN B, et al. Lyocell fibers for textile processing derived from organically grown hemp[J]. Procedia Engineering,2017,200:260−268.
    [3]
    VONAPARTIS E, AUBIN M P, SEGUIN P, et al. Seed composition of ten industrial hemp cultivars approved for production in Canada[J]. Journal of Food Composition and Analysis,2015,39:8−12.
    [4]
    RANALLI P, VENTURI G. Hemp as a raw material for industrial applications[J]. Euphytica,2004,140:1−6.
    [5]
    CALLAWAY J C. Hempseed as a nutritional resource: An overview[J]. Euphytica,2004,140:65−72.
    [6]
    孟妍, 曾剑华, 王尚杰, 等. 汉麻籽蛋白研究进展[J]. 食品工业,2020,41(1):268−273. [MENG Yan, ZENG Jianhua, WANG Shangjie, et al. Research progress of hemp seed protein[J]. The Food Industry,2020,41(1):268−273.
    [7]
    WANG Qingling, JIANG Jiang, XIONG Youling. High pressure homogenization combined with pH shift treatment: A process to produce physically and oxidatively stable hemp milk[J]. Food Research International,2018,106:487−494.
    [8]
    赵越, 魏国江, 潘冬梅, 等. 汉麻籽的营养保健功能及其健康食品发展前景[J]. 黑龙江科学,2015,6(5):12−14. [ZHAO Yue, WEI Guojiang, PAN Dongmei, et al. Nutrition health care function and health food development prospect of hemp seeds[J]. Heilongjiang Science,2015,6(5):12−14.
    [9]
    HAYWARD L, MCSWEENEY M B. Acceptability of bread made with hemp ( Cannabis sativa subsp. sativa ) flour evaluated fresh and following a partial bake method[J]. Journal of Food Science,2020,85(9):2915−2922.
    [10]
    REN Yao, LIANG Kai, JIN Yiqiong, et al. Identification and characterization of two novel α-glucosidase inhibitory oligopeptides from hemp ( Cannabis sativa L. ) seed protein[J]. Journal of Functional Foods,2016,26:439−450.
    [11]
    温林凤, 刘果, 宋明月, 等. 汉麻籽生物活性成分及其应用研究进展[J]. 中国果菜,2021,41(2):21−27. [WEN Linfeng, LIU Guo, SONG Mingyue, et al. Research advance on bioactive components and application of hempseed[J]. China Fruit & Vegetable,2021,41(2):21−27.
    [12]
    耿浩, 刘悦, 梁新婷, 等. 热诱导对乳清浓缩蛋白和分离蛋白乳化性及稳定性的影响[J]. 中国食品学报,2016,16(8):71−77. [GENG Hao, LIU Yue, LIANG Xinting, et al. Effect of heat treatment on emulsification and stability of WPC and WPI[J]. Journal of Chinese Institute of Food Science and Technology,2016,16(8):71−77.
    [13]
    THAIPHANIT S, ANPRUNG P. Physicochemical and emulsion properties of edible protein concentrate from coconut (Cocos nucifera L. ) processing by-products and the influence of heat treatment[J]. Food Hydrocolloids,2016,52:756−765.
    [14]
    CÉSAR B D, TRAUDY W, MARCOS O, et al. Food-grade Pickering stabilizers obtained from a protein-rich lupin cultivar (Alu Prot-CGNA®): Chemical characterization and emulsifying properties[J]. Food Hydrocolloids,2019:87.
    [15]
    FENG Yiming, LEE Y. Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water Pickering emulsion[J]. Food Hydrocolloids,2016,56:292−302.
    [16]
    BO Jiao, SHI Aimin, WANG Qiang, et al. High internal phase Pickering emulsions stabilized solely by peanut protein isolate microgel particles with multiple potential applications[J]. Angewandte Chemie,2018,130(30):9418−9422.
    [17]
    LIU Fu, TANG Chuanhe. Soy protein nanoparticle aggregates as pickering stabilizers for oil-in-water emulsions.[J]. Journal of Agricultural and Food Chemistry,2013,61(37):8888−8898.
    [18]
    SUI Xiaonan, BI Shuang, QI Baokun, et al. Impact of ultrasonic treatment on an emulsion system stabilized with soybean protein isolate and lecithin: Its emulsifying property and emulsion stability[J]. Food Hydrocolloids,2017,63:727−734.
    [19]
    王君文, 韩旭, 李田甜, 等. 乳化剂稳定乳液的机理及应用研究进展[J]. 食品科学,2020,41(21):303−310. [WANG Junwen, HAN Xu, LI Tiantian, et al. Mechanism and application of emulsifiers for stabilizing emulsions: A review[J]. Food Science,2020,41(21):303−310.
    [20]
    GALVES C, STONE A K, SZARKO J, et al. Effect of pH and defatting on the functional attributes of safflower, sunflower, canola, and hemp protein concentrates[J]. Cereal Chemistry,2019,96(6):1036−1047.
    [21]
    DAPČEVIĆ-HADNAĐEV T, DIZDAR M, POJIĆ M, et al. Emulsifying properties of hemp proteins: Effect of isolation technique[J]. Food Hydrocolloids,2019,89:912−920.
    [22]
    王庆玲. 汉麻蛋白改性对蛋白功能性质及营养价值的影响研究[D]. 无锡: 江南大学, 2019.

    WANG Qingling. Effects of protein modification on the functionality and nutritional value of hempseed protein[D]. Wuxi: Jiangnan University, 2019.
    [23]
    CHUANG C C, AIQIAN Ye, ANEMA S G, et al. Concentrated Pickering emulsions stabilised by hemp globulin-caseinate nanoparticles: Tuning the rheological properties by adjusting the hemp globulin :  Caseinate ratio.[J]. Food & Function,2020,11(11):10193−10204.
    [24]
    吴劲轩, 夏文银, 夏川林, 等. 磷脂与食品中蛋白质相互作用机制及对蛋白质特性的影响研究进展[J]. 食品工业科技,2020,41(17):345−351. [WU Jinxuan, XIA Wenyin, XIA Chuanlin, et al. Interaction mechanism between phospholipids and food proteins and effect on protein properties: A review[J]. Science and Technology of Food Industry,2020,41(17):345−351.
    [25]
    YIN Shouwei, TANG Chuanhe, CAO Jinsong, et al. Effects of limited enzymatic hydrolysis with trypsin on the functional properties of hemp ( Cannabis sativa L. ) protein isolate[J]. Food Chemistry,2007,106(3):1004−1013.
    [26]
    PEARCE K N, KINSELLA J E. Emulsifying properties of proteins: Evaluation of a turbidimetric technique[J]. Journal of Agriculture and Food Chemistry,1978,26(3):716−723.
    [27]
    李良, 张小影, 朱建宇, 等. 大豆-乳清混合蛋白对O/W乳液稳定性及流变性的影响[J]. 农业机械学报,2019,50(12):372−379. [LI Liang, ZHANG Xiaoying, ZHU Jianyu, et al. Effect of soybean-whey mixed protein on stability and rheological properties of O/W emulsion[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(12):372−379.
    [28]
    XU Xingfeng, ZHONG Junzhen, CHEN Jun, et al. Effectiveness of partially hydrolyzed rice glutelin as a food emulsifier: Comparison to whey protein[J]. Food Chemistry,2016,213:700−707.
    [29]
    王笑宇, 韩东, 陈子净, 等. 超声处理对 β-伴大豆球蛋白乳化性能的影响[J]. 中国食品学报,2018,18(6):153−159. [WANG Xiaoyu, HAN Dong, CHEN Zijing, et al. Influence of ultrasonic treatment on β-conglycinin emulsibility[J]. Journal of Chinese Institute of Food Science and Technology,2018,18(6):153−159.
    [30]
    王健, 徐晔晔, 于洁, 等. pH偏移结合热处理对大豆蛋白柔性与乳化性的影响[J]. 食品科学,2018,39(14):22−28. [WANG Jian, XU Yeye, YU Jie, et al. Effect of pH migration combined with heat treatment on flexibility and emulsification of soybean protein[J]. Food Science,2018,39(14):22−28.
    [31]
    李秋慧, 齐宝坤, 隋晓楠, 等. 大豆分离蛋白-磷脂复合乳化体系乳化及氧化稳定性研究[J]. 中国食品学报,2016,16(5):46−52. [LI Qiuhui, QI Baokun, SUI Xiaonan, et al. Studies on emulsification and oxidation stability of the soybean isolate protein-phospholipid emulsion system[J]. Journal of Chinese Institute of Food Science and Technology,2016,16(5):46−52.
    [32]
    FELIX M, ISURRALDE N, ROMERO A, et al. Influence of pH value on microstructure of oil-in-water emulsions stabilized by chickpea protein flour[J]. Food Science and Technology International,2018,24(7):555−563.
    [33]
    韩天翔, 李杨, 毕爽, 等. 磷脂对大豆乳清蛋白乳化特性的影响[J]. 食品与发酵工业,2017,43(2):13−20. [HAN Tianxiang, LI Yang, BI Shuang, et al. Effect of phospholipids on the emulsifying properties of soy whey protein[J]. Food and Fermentation Industries,2017,43(2):13−20.
    [34]
    李良, 周艳, 邹智博, 等. 高温豆粕大豆分离蛋白射流空化辅助提取[J]. 农业机械学报,2019,50(9):373−380. [LI Liang, ZHOU Yan, ZOU Zhibo, et al. Extraction of soy protein isolate from highly denatured soybean meal by jet cavitation assisted[J]. Journal of the Chinese Society for Agricultural Machinery,2019,50(9):373−380.
    [35]
    BERTON-CARABIN C C, SCHROËN K. Pickering emulsions for food applications: Background, trends, and challenges[J]. Annual Review of Food Science and Technology,2015,6(1):263−297.
    [36]
    LI Songnan, ZHANG Bin, TAN C P, et al. Octenylsuccinate quinoa starch granule-stabilized Pickering emulsion gels: Preparation, microstructure and gelling mechanism[J]. Food Hydrocolloids,2019,91:40−47.
    [37]
    TORRES L G, ITURBE R, SNOWDEN M J, et al. Preparation of o/w emulsions stabilized by solid particles and their characterization by oscillatory rheology[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2007,302(1):439−448.
    [38]
    葛慧娟, 肖军霞, 黄国清. 乳清分离蛋白-果胶复合物在Pickering乳液制备中的应用[J/OL]. 中国食品学报: 1−15[2021-03-10]. http://kns.cnki.net/kcms/detail/11.4528.TS.20210302.0902.002.html.

    GE Huijuan, XIAO Junxia, HUANG Guoqing. The application of whey protein isolate-pectin complex in the preparation of Pickering emulsions [J/OL]. Journal of Chinese Institute of Food Science and Technology: 1−15[2021-03-10]. http://kns.cnki.net/kcms/detail/11.4528.TS.20210302.0902.002.html.
    [39]
    武飞扬, 古月, 马鸣阳, 等. 水包油食品乳液中天然小分子乳化剂的功能性质及与其他乳化剂相互作用关系的研究进展[J/OL]. 食品科学: 1−15[2021-04-10]. http://kns.cnki.net/kcms/detail/11.2206.TS.20201228.1710.050.html.

    WU Feiyang, GU Yue, MA Mingyang, et al. Research progress on the functional properties of natural small molecule emulsifiers in oil-in-water food emulsions and their interaction with other emulsifiers [J/OL]. Food Science: 1−15 [2021-04-10]. http://kns.cnki.net/kcms/detail/11.2206.TS.20201228.1710.050.html.
    [40]
    曾剑华, 孟妍, 刘琳琳, 等. 基于多光谱技术表征汉麻分离蛋白构象并分析pH对其构象的影响[J]. 光谱学与光谱分析,2020,40(12):3748−3754. [ZENG Jianhua, MENG Yan, LIU Linlin, et al. The conformation of hemp protein isolate was characterized by multispectral technique and the influence of pH on its conformation was analyzed[J]. Spectroscopy and Spectral Analysis,2020,40(12):3748−3754.

Catalog

    Article Metrics

    Article views (254) PDF downloads (18) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return