Isolation, Purification and Antioxidant Activity of <i> Inonotus obliquus </i>Polysaccharide

LI Xinxin; LI Wenxiang

doi:10.13386/j.issn1002-0306.2020040211

Volume 42 Issue 11

May 2021

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Science and Technology of Food Industry > 2021 > 42(11): 192-197. > DOI: 10.13386/j.issn1002-0306.2020040211

LI Xinxin, LI Wenxiang. Isolation, Purification and Antioxidant Activity of Inonotus obliquus Polysaccharide[J]. Science and Technology of Food Industry, 2021, 42(11): 192−197. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020040211.

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Isolation, Purification and Antioxidant Activity of Inonotus obliquus Polysaccharide

LI Xinxin,
LI Wenxiang^,

School of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China

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Received Date: April 19, 2020
Available Online: April 06, 2021

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Abstract

The chemical compositions of Inonotus obliquus were analyzed and crude polysaccharides (IOP)were isolated from Inonotus obliquus by water extraction. The component of polysaccharides were purified by DEAE-52, and Sephadex G-100 column chromatography, and the highly active polysaccharide component was screened out through antioxidant activity, and the purity of the polysaccharide component was analyzed by high-performance liquid chromatography.The experimental results showed that the chemical composition of Inonotus obliquus was rich, including polysaccharide 13.10%± 0.31%, reducing sugar 4.21%±0.40%, protein 2.70%±0.71%, ash 9.60%±0.31%.After IOP undergoes DEAE-52 cellulose column chromatography, four monosaccharide components were obtained: IOP-1, IOP-2, IOP-3, and IOP-4. Antioxidation experiments on four polysaccharide components found that IOP-2 had the highest scavenging rate for DPPH free radicals. After passing through Sephadex-100 chromatography column, IOP-2 obtained two polysaccharide components: IOP-2a and IOP-2b. Compared with its antioxidant activity, the polysaccharide component with higher activity was selected as IOP-2a. Using high performance liquid chromatography to identify the polysaccharide component IOP-2a, only one symmetrical peak was detected, indicating that IOP-2a was a homogeneous polysaccharide.
- Inonotus obliquus,
- polysaccharide,
- separation and purification,
- antioxidant,
- high performance liquid chromatography

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Isolation, Purification and Antioxidant Activity of Inonotus obliquus Polysaccharide

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