Fermentation Conditions Optimization of a Two-stage Culture for Enhancing Production of Lycopene by Neurospora crassa Using Response Surface Methodology

The effect of two-stage culture method(by combing shake culture with static culture)on lycopene production was studied in this study. Through Box-behnken experimental design and response surface methodology,the optimum conditions were shake culture 19 h,static culture 87 h,the concentration of medium 35 g/L,inoculum size of 5%,culture temperature 30℃ and bottle shaking rate 100 r/min. Under this conditions,the dry cell weight decreased by 23.54% and lycopene production increased by 102.54% compared with conventional shake culture.