Optimization of Extraction Technology of Water-soluble Flavonoids and Total Polysaccharides from Aurea helianthus Flower by Response Surface Methodology

Based on the single-factor-test,the response surface methodology (RSM) was employed to obtain the optimum extraction conditions,including extraction methods,extraction time,extraction temperature and solid/liquid ratio with the contents of water-soluble flavonoids and polysaccharide from Aurea helianthus flower as the response indexes. And the composition of flavonoids and polysaccharides was studied by high performance liquid chromatography (HPLC).The results showed that compared with other methods,the soak extraction was simple in operation and low in cost,and avoided damage to biological active ingredients by heating for a long time. The optimized extraction conditions of water-soluble flavonoids and polysaccharide were as follows:Extraction time of 240 min,extraction temperature of 90℃ and solid/liquid ratio of 1:270. Under these conditions,contents of total flavonoids and polysaccharide of Aurea helianthus flowers reached (43.23±0.56), (134.60±1.45) mg/g,which closely agreed with the predicted values,and the relative errors were 0.44% and 0.69%,respectively.The flavonoids in the Aurea helianthus flower were mainly composed of rutin,hyperoside,isoquercitrin,hibifolinand myricetin,in which the content of hibifolin was the highest. The polysaccharide mainly consisted of rhamnose,glucose and galactose,with a small amount of fucose,arabinose and mannose.