Determination of Thirteen Pesticides in Edible Mushrooms by QuEChERS with UPLC MS/MS

Objective:Determination of thirteen pesticides in edible mushrooms by QuEChERS with UPLC MS/MS. Methods:5.0 g sample was extracted ultrasonically for 5 min with 15 mL of 4:1 acetonitrile and water. The extract was purified by N-propyl ethylenediamine (PSA) 30.0 mg, anhydrous Magnesium sulfate 15.0 mg dispersive solid-phase extraction adsorbent.The purified solution was separated by UPLC using HSS T3 column, 0.1% formic acid and acetonitrile as mobile phase in gradient elution. Positive ESI and MRM were adopted in MS analysis. Results:Linear relationships were found between the peak area and the mass concentration of 13 pesticides within 0.5~200 μg/L. With detection limits (S/N=3) in the range of 0.03~0.3 μg/kg, recovery rates obtained by standard addition method ranged from 84.3% to 102% and RSDs (n=6) were in the range of 2.8%~6.3%. Conclusion:The proposed method was successfully applied to the detection of 13 pesticides in mushrooms.