Optimization of Mechanical Grinding Assisted Extraction of Proanthocyanidins from Shenzhou Jujube By Response Surface Methodology

This study was aimed to optimize the mechanical grinding assisted extraction(MGAE)of proanthocyanidins from Shenzhou jujube. The optimization was performed using one-factor-at-a-time(OFAT)method and response surface methodology(RSM). The results showed that the optimum conditions were as follows:grinding material particle size 3 mm,grinding time 16 min,grinding material dosage(volume ratio)30%,grinding temperature 62 ℃,liquid material ratio 25 mL/g,the volume fraction of ethanol 61%. Under the optimized process conditions,the yield of proanthocyanidins in the leaves was 1.942%±0.001%.The actual verification experiment showed that the relative error between the predicted maximum regression rate and the verification test yield of the regression equation model was 0.052%,and the fitted function model was available. Experiments proved that the process of mechanical grinding extraction of proanthocyanidins from Shenzhou jujube was feasible.