Anti-inflammatory Effects of Antrodia cinnamomea Ethanol Extract in Vitro

Objective:To investigate the anti-inflammtory effects of ethanol extract from Antrodia cinnamomea(AC-E)on the T cell and T cell subsets,exploring the anti-inflammatory effect of Antrodia cinnamomea. Methods:Splenocytes from BAL B/C mice were treated with AC-E(12.5,25,50 μg/mL)under the stimulation by ConA(3 μg/mL)for 72 h. Cell vitality was measured by MTS assay. Apoptosis was determined with flowcytometry(FCM),as well as T cells and subsets. Concentrations of interferon-γ(IFN-γ)and tumor nerosis factor-α(TNF-α)in the supernatant were determined by enzyme linked immunosorbent assay(ELISA). Results:Data showed that the AC-E(50 μg/mL)could directly inhibit the proliferation of splenocytes under ConA stimulation. The cells in AC-E(25,50 μg/mL)dose groups showed obvious apoptosis. Results of ELISA showed that AC-E could suppress the secretions of TNF-α and IFN-γ,and the concentration of cytokines in the drug-treatment groups were close to that of normal group. Conclusion:AC-E could restore the immune system via apoptosis inducion in activated T cells,providing experimental basis and theoretical basis for anti-inflammatory application of Antrodia cinnamomea.