Identification of Spore-Forming Strains Isolated from Clean Environment and Its Drug Resistance
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1. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;
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2. Shanghai Nod Bio Technology Co., Ltd., Shanghai 201306, China;
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3. Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, China;
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4. National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai), Shanghai 201306, China;
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5. National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China
More Information
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- Received Date:
September 24, 2018
- Available Online:
November 12, 2020
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Abstract
In order to study the species and drug resistance of spore-forming bacteria,settled bacteria was selected from clean environment by the national standard(GB/T 16294-2010). The spore-forming strains were screened from the purified settled bacteria by the method of Schaeffer-Fulton’s spore dyeing. The 16S rDNA sequence alignment analysis was used to identify the strains. The method of K-B was used to detect the resistance of the strains to 20 antibiotics. The results showed that 74 strains were collected and 14 spore-forming strains were detected in different clean environments(pharmaceutical,clean bench,cell room),and the detection rate of spore-forming strains in clean environments was 18.9%. At the same time,it was found that its resistance rate to β-lactam antibiotics was 92.86%. It had shown that spore-forming strains in the clean environment had certain resistance and provided reference data for the safety risk of spore-forming strains in clean environment.
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References
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