Antioxidant Activities and Analsis of Active Components in Different Extract Phases of Chamomile

Objective:The purpose was to obtain the extraction fraction with the strongest antioxidant activity of the chamomile alcohol extracts,and thereby to explore its active components. Methods:The methods such as DPPH·,ABTS⁺·,O₂^-· and FRAP were used to compare the antioxidant activities of different polar fractions of chamomile alcohol extracts. And then,the compositions of the strongest antioxidant fraction were analyzed by HPLC. Results:The ethyl acetate fraction of chamomile alcohol extracts had the strongest antioxidant capacity. The IC₅₀ of ethyl acetate fraction for DPPH·,ABTS⁺· and O₂^-· were 25.6,30.6 and 83.3 mg/mL respectively,and the total antioxidant capacity(presented as trolox)was 16.7 mg/mL. The composition analysis showed that the contents of total phenol and total flavonoid in the ethyl acetate fraction were 25.84% and 14.93%,respectively. And the contents of luteolin,glucoside and luteolin-7-glucose were the highest among the 10 polyphenols or flavonoids in the ethyl acetate phase. Conclusion:The good antioxidant activity of acetate extracts of the extract of chamomile alcohol might be related to its rich polyphenols and flavonoids.