Quantitative Detection of Ovine-derived Materials in Meat Products by Real-time Fluorescent PCR

In order to determine the contents of ovine in meat products,primers and probes were designed with their conservative regions of single copy gene to prepare recombinant plasmids for the universal gene of sheep and vertebrate to construct a standard curve. A fluorescent quantitative PCR detection system was established by calculating the number of copies by the equation,calculating the percentage contents of the mutton source in the sample based on the ratio of the copy number and the mixed meat samples with the known target meat content were quantitatively detected. The results showed that the PCR method had good specificity and sensitivity,and the minimum DNA contents of ovine was 0.02 ng/μL. Quantitative detection with a known target meat showed that the results were relatively accurate when the incorporation was 90% and 50%,and the relative standard deviations were 5.69% and 10.82%, respectively. These results were not interfered by exogenous species. The quantitative PCR method established in this experiment could accurately detect the contents of mutton in different samples and find the adulteration.