Diversity of Lactic Acid Bacteria from Suanjiangshui in Zaoyang and Its Evaluation of Isolation Method

DGGE was used to analyze the bacterial community of Suanjiangshui samples, and the microbial compositions in Suanjiangshui were separated and identified by six common lactic acid bacteria separation methods, and the separation effects of these methods were evaluated. The results showed that there were differences in number of lactic acid bacteria among the Suanjiangshui from different sources. The number of lactic acid bacteria were about 5×10⁶~2.5×10⁸ cfu/mL. A total of 90 strains of lactic acid bacteria were obtained, which were identified as Lactobacillus delbrueckii, Lactobacillus fermentium, Lactobacillus zeae, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus paracasei. Among the methods used in this study, anaerobic jar with anaeropack, anaerobic workstation (10% H₂, 10% CO₂, 80% N₂) and enumeration by litmus milk medium method were the best methods for LAB isolation.