Label-free Quantitative Proteomic Analysis of Acipenser schrenki Brandt Peptides on Aging Mice Induced by D-galactose

To investigate anti-aging mechanism of A. schrenki Brandt peptides (ASP), label-free quantitative proteomics was used. The results of LFQ (Label Free Quantization) showed 442 low-abundant proteins in serum in mice treated with ASP. These proteins covered 21 biological processes, 7 molecular functions and 10 cellular components. There were 3 differentially expressed proteins according to t-test. In bioinformatics analysis, the screening criteria were expanded to 9 proteins. Among the 9 proteins, the up-regulated bisphosphoglycerate mutase (AC:P15327), apolipoprotein D (AC:P51910), flavin reductase (AC:Q923D2) and peroxiredoxin-2 (AC:Q61171) and down-regulated sulfhydryl oxidase 1, (AC:Q8BND5) could improve the ability to scavenge free radicals. These results showed that the ASP prevented aging by scavenging free radicals.