Optimization of Microwave-assisted Extraction of Anthocyanins from Lonicera edulis and Its Antioxidant Activity by Response Surface Methodology

In order to make full use of wild Lonicera edulis and improve its value, the microwave-assisted extraction of anthocyanin from Lonicera edulis and its antioxidant activity in vitro were studied. In this paper, the wild Lonicera edulis fruit in Xiaoxingan Mountains was used as the experimental raw material. Optimization of anthocyanin extraction from Lonicera edulis by Response Surface Methodology, the scavenging ability to 3 kinds of free radicals and total reducing power were also analyzed. The results showed that microwave assisted extraction of anthocyanins from Lonicera edulis anthocyanins was microwave power 280 W, microwave time 90 s, material liquid ratio 1:25 g/mL, and ethanol volume fraction 85%. Under the conditions, the extraction of anthocyanin of Lonicera edulis was (292.16±1.25) mg/100 g. The anthocyanins of Lonicera edulis have a certain antioxidant capacity, and have high scavenging ability for DPPH free radical, ABTS⁺·and superoxide anion radical.The scavenging rate was 89.2%, 70.4% and 44.73% respectively, and the total reduction ability was higher. This study provided an economical and feasible method for extracting anthocyanins from Lonicera edulis and further explored the value of Lonicera edulis.