Bactericidal and Inhibitory Effect of Different Calcined Shell Powder

Objective:to study the bactericidal and inhibitory effect of different calcined shell powder on pathogenic bacteria. Methods:Shells of Mussels,Meretrix meretrix and Sinonovacula constricta were calcined at a high temperature of 1000℃ for 2 h,and diluted into 1%,0.1%,0.01% concentration of calcination powder suspension. The bactericidal and inhibitory effect of 3 kinds of calcined shell powder on Salmonella,Vibrio parahaemolyticus,Staphylococcus aureus and Escherichia coli were detected using bacteria sterilization and culture medium diffusion method. Results:The calcined shell powder had bactericidal effects on 4 kinds of bacteria,and gram negative bacteria better than gram positive bacteria. Sinonovacula constricta and Meretrix meretrx calcined shell powder on Vibrio parahaemolyticus was the strongest,the number of bacteria was less than 10 CFU/mL after 5 min.The inhibitory experiment of the medium diffusion method showed that 3 kinds of calcined shell powder had inhibitory effect on 4 kinds of bacteria. Under the concentration of 0.1 g/mL,the inhibitory effect of Sinonovacula constricta calcined shell powder on Salmonella was the strongest and the diameter of inhibition zone was 16.42 mm. At each concentration,the inhibition of Mussels and Meretrix meretrix shell calcination powder on Vibrio parahaemolyticus was significantly stronger than that of Salmonella and Escherichia coli. Conclusion:The calcined shell powder as a inhibitor had obvious bactericidal and inhibitory effect on food borne pathogens.