Extraction,Purification and Stability of Anthocyanin from Mulberry

Anthocyanin from mulberry was extracted by ultrasonic assisted solvent method. According to adsorption rate and desorption rate,this paper selected good performance macroporous resin from HPD600,D101,LX1180,AB-8,DA201-C for purification,and the purified material's stability were studied. The results showed that the extraction rate of anthocyanin was 2.9 mg/g when 40% of ethanol(including 0.1% HCl)was used as extracting agent with a sample/solvent ratio 1:10,and the ultrasonic time 90 min. Among macroporous resins selected,the HPD600 was the best for the purification of anthocyanin. The sugar-removing ratio was 67.6%,the purity was 16.7%,the color value was 46.1,and the product was purple black powder. It contained about 41.2% of cornflower-3-O-glucoside. With the increasing of temperature,pH and illumination intensity,the stability of anthocyanin decreased. Anthocyanin was sensitive to Cu⁺,Cu²⁺,Fe²⁺,Fe³⁺,while Na⁺,Mg²⁺,Al³⁺ had a little influence on the stability of anthocyanins. Purified anthocyanin should be processed and stored in conditions of 25℃,low pH value,the dark and avoid contact cupric ion or iron ion.