Effect of Slurry Ice Storage Combined with High Hydrostatic Pressure Treatment on the Microflora Analysis of Refrigerated Pseudosciaena crocea

The use of slurry ice,both alone and in combination with high hydrostatic pressure processing as compared with traditional flake ice was investigated as preservation method for Pseudosciaena crocea. The diversity of dominant spoilage bacteria in Pseudosciaena crocea were monitored by the combination of 16S rDNA PCR amplification with physiological and biochemical identification. The results showed that the dominating bacteria were Shewanella spp during the ice storage. Slurry ice storage inhibited the growth of microorganisms,the total microbial count was lower than the same period the iced group. At the end of shelf life some salt resistant gram positive microorganisms such as Micrococcusroseus(19.4%),lactic acid bacteria(2.9%)and Staphylococcus spp. (4.3%)occupied a certain proportion,this may related to the salt component in slurry ice.The application of high pressure processing decreased the total microbial count of 0.4 lg CFU/g in fish muscle and had stronger effect on gram negative micro organism,combined with slurry ice storage inhibited the microorganisms which had stronger ability of causing corruption that has a potential applying in preservation of aquatic products.