Optimization of cryoprotectants formulation for Pediococcus acidilactici by response surface methodology

The addition of freeze-drying cryoprotectants can improve the survival rate of microbial effectively. In order to increase the survival rate, cryoprotectants for Pediococus acidilactici were investigated using response surface methodology joined by Plackett Burman design and steepest ascend method. The results showed that trehalose, mannitol and manganese sulfate significantly affect the survival rate of Pediococus acidilactici. The interaction between trehalose and mannitol was remarkable, manganese sulfate did not interact with trehalose and mannitol remarkablely. The optimal amounts of cryoprotectants fomula were trehalose 73.64 g/L, mannitol 43.76 g/L, manganese sulfate 1.91 g/L and the survival rate reached 98.10%±1.03%. The experimental results provided a technical support for the production of probiotics.