Optimization of fermentation medium and culture conditions of producing bacterial cellulose of Gluconacetobacter hensennii strain

Single factors tests combined response surface design were carried out to optimize fermentation medium of producing bacterial cellulose of Gluconacetobacter hensennii strain. The strain was isolated by our laboratory from traditional kombucha. Besides,based on the optimized medium,culture conditions were optimized by single factors tests and orthogonal experiments. The results showed that the optimal medium(g/L)contained 10 g sucrose,9.36 g yeast extract powder,5 g peptone,2.86 g disodium hydrogen phosphate,0.3 g citric acid monohydrate,pH5.96. Under the condition,BC dry weight was 4.779 g/L. Based on the medium,performed fermenting progress in 50 mL medium with 10% seed solution at 30℃ for 10 d. Under the condition,BC dry weights could arrived 8.515 g/L and the growth rate was more than 300% compared to control group.