Bacteriostatic effects of different spices essential oils on Clostridium perfringens in vitro

WANG Xiao-hui; WEI Fa-shan; LI Miao-yun

doi:10.13386/j.issn1002-0306.2017.23.003

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Science and Technology of Food Industry > 2017 > (23): 10-13. > DOI: 10.13386/j.issn1002-0306.2017.23.003

WANG Xiao-hui, WEI Fa-shan, LI Miao-yun. Bacteriostatic effects of different spices essential oils on Clostridium perfringens in vitro[J]. Science and Technology of Food Industry, 2017, (23): 10-13. DOI: 10.13386/j.issn1002-0306.2017.23.003

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Bacteriostatic effects of different spices essential oils on Clostridium perfringens in vitro

College of Food Science and Technology, Henan Agricultural University
Product Quality Supervision and Inspection Institute of Henan Province

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Received Date: March 05, 2017

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The effect of different spices essential oils (angelica essential oil, black-pepper essential oil, anise essential oil, cinnamon essential oil, ginger essential oil and wormwood essential oil) on the growth of Clostridium perfringen (C.perfringens) was studied to select the spices essential oils which had inhibitory effect on Clostridium perfringens.Oxford cup method and two times dilution method were selected to determine the antibacterial circle and minimum inhibitory concentration (MIC) for C.perfringens (Standard strain ATCC13124 and CICC22949, isolated strain C₁and C₂) .The result showed that the diameter of antibacterial circle of cinnamon essential oil was the largest, and the minimum inhibitory concentration (MIC) of cinnamon essential oil on C.perfringens ATCC13124, CICC22949, C₁and C₂were 2.75, 2.75, 5.5 and 2.75 mg/m L, respectively.The minimum inhibitory concentration of wormwood essential oil on C.perfringens ATCC13124, CICC22949, C₁and C₂were 11, 2.75, 11 and 2.75 mg/m L, respectively.The minimum inhibitory concentration of black-pepper essential oil on C.perfringens ATCC13124, CICC22949, C₁and C₂were 22, 11, 11 and 5.5 mg/m L, respectively.The minimum inhibitory concentration of anise essential oil on C.perfringens ATCC13124, CICC22949, C₁and C₂were 11, 5.5, 11 and 2.75 mg/m L, respectively.The minimum inhibitory concentration of ginger essential oil on C.perfringens ATCC13124, CICC22949, C₁and C₂were 88, 22, 22 and22 mg/m L, respectively.Conclusion:In addition to angelica essential oil, other five spices essential oils had inhibitory effect on C.perfringens, and the inhibition effect of cinnamon essential oil on C.perfringens was the best.
- spices essential oils,
- Clostridium perfringens,
- antibacterial circle,
- minimum inhibitory concentration (MIC)

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[1]	陈小云, 关孚时, 张存帅, 等.产气荚膜梭菌主要外毒素最新研究进展[J].中国兽药杂志, 2005, 39 (6) :29-33.
[2]	区敏港, 彭新宇, 唐兴刚, 等.黑胡椒、快乐鼠尾草与生姜精油的化学成分及对产气荚膜梭菌的抑制活性研究[C].中国畜牧兽医学会中兽医学分会2011年学术年会、中南六省区中西兽医结合研究会广东省中兽药产业发展创新论坛, 2011.
[3]	李凤清.植物精油的抑菌评价及其应用[D].南京:南京师范大学, 2014.
[4]	赵鑫, 鲍其泠, 王改香.薰衣草精油成分分析及其抗菌和抗氧化活性研究[J].日用化学品科学, 2013, 36 (4) :32-34, 43.
[5]	刘晓艳, 白卫东.肉桂油抑菌及抗氧化作用的研究进展[J].食品与机械, 2010, 26 (5) :169-172.
[6]	钟瑞敏, 王羽梅, 曾庆孝, 等.芳香精油在食品保藏中的应用性研究进展[J].食品与发酵工业, 2005, 31 (3) :93-98.
[7]	李京晶, 籍保平, 周峰, 等.丁香和肉桂挥发油的提取、主要成分测定及其抗菌活性研究[J].食品科学, 2006, 27 (8) :64-68.
[8]	顾仁勇, 张石峰, 刘莹莹, 等.五种香辛料精油抑菌及抗氧化性能研究[J].食品科学, 2008, 29 (3) :106-108.
[9]	Deans S G, Ritchie G.Antibacterial properties of plant essential oils[J].International Journal of Food Microbiology, 1987, 5 (2) :165-180.
[10]	López P, Sánchez C, Batlle R, et al.Solid-and vapor-phase antimicrobial activities of six essential oils:susceptibility of selected foodborne bacterial and fungal strains.[J].Journal of Agricultural&Food Chemistry, 2005, 53 (17) :6939-6946.
[11]	Tassou C C, Drosinos E H, Nychas G J.Effects of essential oil from mint (Mentha piperita) on Salmonella enteritidis and Listeria monocytogenes in model food systems at 4 degrees and 10degrees C.[J].Journal of Applied Microbiology, 1995, 78 (6) :593-600.
[12]	Khalfallah A, Labed A, Semra Z, et al.Antibacterial activity and chemical composition of the essential oil of Ammi visnaga L. (Apiaceae) from Constantine, Algeria.[J].International Journal of Medicinal&Aromatic Plants, 2011, 1 (3) :302-305.
[13]	阳艳林, 肖建根.不同枯草芽孢杆菌制剂对产气荚膜梭菌的抑制作用[J].中国畜牧杂志, 2011, 47 (12) :55-56.
[14]	刘冬梅, 李理, 杨晓泉, 等.用牛津杯法测定益生菌的抑菌活力[J].食品研究与开发, 2006, 27 (3) :110-111.
[15]	佘之蕴, 黄宝莹, 刘海卿, 等.牛津杯法测定食品添加剂对五种益生菌的抑菌活力[J].食品工业, 2016 (1) :171-174.
[16]	黄宝莹, 佘之蕴, 苏妙仪, 等.食品添加剂对两种乳酸菌的抑菌作用[J].中国乳品工业, 2015, 43 (8) :16-18.
[17]	顾仁勇, 张丽, 傅伟昌, 等.芭蕉汁的抑菌作用[J].食品与发酵工业, 2005, 31 (3) :57-59.
[18]	蔡一鸣, 任荣清, 文正常.中药方剂的抑菌实验[J].贵州畜牧兽医, 1995 (4) :4-5.
[19]	邹君彪, 黄苓, 冷董碧.不同抗生素对产气荚膜梭菌的抑菌效果研究[J].国外畜牧学-猪与禽, 2013, 33 (6) :103-106.
[20]	马绪荣.药品微生物学检验手册[M].北京:科学出版社, 2000:211-212.
[21]	赵丹, 孙旸, 陈光.肉桂精油的抑菌作用[J].吉林农业大学学报, 2013, 35 (4) :402-405.
[22]	王步江, 刘金福, 樊秀花, 等.肉桂精油抑菌活性研究[J].食品与机械, 2011 (6) :166-167.
[23]	柴向华, 董艳, 吴克刚, 等.植物精油对食品中常见有害微生物的抑菌活性研究[J].现代食品科技, 2016 (8) :123-127.

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