Isolation, identification and enzymology properties of an agarase-producing strain

An agar-decomposing strain was isolated from Qingdao offshore deposits. The optimal fermentation time was 26 h, transparent zones appeared after staining with lugol 's iodine. The bacteria was gram-negative and classified in the genus Acinetobacter, named as Acinetobacter sp.LXK.By studying the primary properties of agarase and study showed that: the optimal p H and temperature for enzymolysis were p H8.0 and 40 ℃ respectively, the optimum concentration of substrate was 0.5%, the enzyme was activate by K⁺, Ca² +and inhabited by Fe ⁽2+）, Mn^（2+）, Ba^（2+）.