Citation: | CHEN Yi-chun, NIU Hai-li, KONG Bao-hua. Application of quantitative real-time PCR in meat and meat products[J]. Science and Technology of Food Industry, 2017, (17): 324-328. DOI: 10.13386/j.issn1002-0306.2017.17.063 |
[1] |
Toorop R M, Murch S J, Ball R O.Development of a rapid and accurate method for separation and quantification of myofibrillar proteins in meat.[J].Food Research International, 1997, 30 (8) :619-627.
|
[2] |
Arun O O, Ugˇur M.Animal Species Determination in Sausages using an SDS-PAGE technique[J].2000, 51 (2) :49-53.
|
[3] |
Doi H, Watanabe E, Shibata H, et al.A Reliable Enzyme Linked Immunosorbent Assay for the Determination of Bovine and Porcine Gelatin in Processed Foods[J].J Agric Food Chem, 2009, 57 (5) :1721-1726.
|
[4] |
Rohman A, Sismindari, Erwanto Y, et al.Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy[J].Meat Science, 2011, 88 (1) :91-95.
|
[5] |
赵洁, 马晨, 席晓敏, 等.实时荧光定量PCR技术在肠道微生物领域中的研究进展[J].生物技术通报, 2014 (12) :61-66.
|
[6] |
Kim J, Lim J, Lee C.Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems:applications and considerations.[J].Biotechnology Advances, 2013, 31 (8) :1358-1373.
|
[7] |
严菊英, 卢亦愚, 冯燕, 等.Taq Man荧光定量RT-PCR快速检测甲3型流感病毒[J].中国人兽共患病学报, 2005, 21 (2) :169-172.
|
[8] |
Kadmiri N E, Khachibi M E, Slassi I, et al.Assessment of GAPDH expression by quantitative real time PCR in blood of Moroccan AD cases[J].Journal of Clinical Neuroscience, 2017, 40:24-26.
|
[9] |
Sthlberg A, Bengtsson M.Single-cell gene expression profiling using reverse transcription quantitative real-time PCR[J].Methods, 2010, 50 (4) :282-288.
|
[10] |
Zhong Q, Bhattacharya S, Kotsopoulos S, et al.Multiplex digital PCR:Breaking the one target per color barrier of quantitative PCR[J].Lab on A Chip, 2011, 11 (13) :2167-2174.
|
[11] |
Bassam B J, Allen T, Flood S, et al.Nucleic acid sequence detection systems:Revolutionary automation for monitoring and reporting PCR products[J].Australasian Biotechnology, 1996, 6 (5) :285-294.
|
[12] |
刘小荣, 张笠, 王勇平.实时荧光定量PCR技术的理论研究及其医学应用[J].中国组织工程研究, 2010, 14 (2) :329-332.
|
[13] |
Alkahtani H A, Ismail E A, Asif A M.Pork detection in binary meat mixtures and some commercial food products using conventional and real-time PCR techniques[J].Food Chemistry, 2016, 219:54-60.
|
[14] |
Killgore G E, Holloway B, Tenover F C.A 5'nuclease PCR (Taq Man) high-throughput assay for detection of the mec A gene in staphylococci[J].Journal of Clinical Microbiology, 2000, 38 (7) :2516-2519.
|
[15] |
Gibson N J.The use of real-time PCR methods in DNA sequence variation analysis[J].Clinica Chimica Acta, 2006, 363 (1-2) :32-47.
|
[16] |
Sentandreu Má, Sentandreu E.Authenticity of meat products:Tools against fraud[J].Food Research International, 2014, 60 (6) :19-29.
|
[17] |
Myers M A.Direct measurement of cell numbers in microtitre plate cultures using the fluorescent dye SYBR green I[J].Journal of Immunological Methods, 1998, 212 (1) :99-103.
|
[18] |
Siddiqui S, Khan I, Zarina S, et al.Use of the SYBR Green dye for measuring helicase activity[J].Enzyme&Microbial Technology, 2013, 52 (3) :196-198.
|
[19] |
Wang W, Chen K, Xu C.DNA quantification using Eva Green and a real-time PCR instrument[J].Analytical Biochemistry, 2006, 356 (2) :303-305.
|
[20] |
Fajardo V, González I, Rojas M, et al.A review of current PCR-based methodologies for the authentication of meats from game animal species[J].Trends in Food Science&Technology, 2010, 21 (8) :408-421.
|
[21] |
Mohamad N A, Sheikha A F E, Mustafa S, et al.Comparison of gene nature used in real-time PCR for porcine identification and quantification:A review[J].Food Research International, 2013, 50 (1) :330-338.
|
[22] |
E Unionlegislation..Council regulation (EC) //No 178/2002of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety[J].Official Journal European Communities I, 2002, 31:1-24.
|
[23] |
Soares S, Amaral J S, Oliveira M B, et al.A SYBR Green real-time PCR assay to detect and quantify pork meat in processedpoultry meat products[J].Meat Science, 2013, 94 (1) :115-120.
|
[24] |
Ballin N Z, Vogensen F K, Karlsson A H.Species determination-Can we detect and quantify meat adulteration[J].Meat Science, 2009, 83 (2) :165-174.
|
[25] |
Haunshi S, Basumatary R, Girish P S, et al.Identification of chicken, duck, pigeon and pig meat by species-specific markers of mitochondrial origin[J].Meat Science, 2009, 83 (3) :454-459.
|
[26] |
Fajardo V, González I, Martín I, et al.Differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR analysis targeting the mitochondrial D-loop and the nuclear melanocortin receptor 1 (MC1R) genes[J].Meat Science, 2008, 78 (3) :314-322.
|
[27] |
Barakat H, El-Garhy H A S, Moustafa M M A.Detection of pork adulteration in processed meat by species-specific PCRQIAxcel procedure based on D-loop and cytb, genes[J].Applied Microbiology and Biotechnology, 2014, 98 (23) :9805-9816.
|
[28] |
Martín I, García T, Fajardo V, et al.SYBR-Green real-time PCR approach for the detection and quantification of pig DNA in feedstuffs[J].Meat Science, 2009, 82 (2) :252-259.
|
[29] |
Kim M, Yoo I, Lee S Y, et al.Quantitative detection of pork in commercial meat products by Taq Man;real-time PCR assay targeting the mitochondrial D-loop region[J].Food Chemistry, 2016, 210:102-106.
|
[30] |
Dooley J J, Paine K E, Garrett S D, et al.Detection of meat species using Taq Man real-time PCR assays[J].Meat Science, 2004, 68 (3) :431-438.
|
[31] |
Ali M E, Hashim U, Dhahi T S, et al.Analysis of Pork Adulteration in Commercial Burgers Targeting Porcine-Specific Mitochondrial Cytochrome B Gene by Taq Man Probe Real-Time Polymerase Chain Reaction[J].Food Analytical Methods, 2012, 91 (4) :454-459.
|
[32] |
Ali M E, Hashim U, Mustafa S, et al.Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by Taq Man probe real-time polymerase chain reaction[J].Food Analytical Methods, 2012, 91 (4) :454-459.
|
[33] |
Filonzi L, Chiesa S, Vaghi M, et al.Molecular barcoding reveals mislabelling of commercial fish products in Italy[J].Food Research International, 2010, 43 (5) :1383-1388.
|
[34] |
Fang X, Zhang C.Detection of adulterated murine components in meat products by Taq Manreal-time PCR[J].Food Chemistry, 2016, 192:485-490.
|
[35] |
Belloque J, García M C, Torre M, et al.Analysis of soyabean proteins in meat products:a review[J].Critical Reviews in Food Science and Nutrition, 2002, 42 (5) :507-532.
|
[36] |
李碧晴, 余坚勇, 盛东飚, 等.大豆组织蛋白在猪肉丸中的应用[J].肉类研究, 2001 (4) :33-35.
|
[37] |
Castro F, Garcia M C, Rodriguez R, et al.Determination of soybean proteins in commercial heat-processed meat products prepared with chicken, beef or complex mixtures of meats from different species[J].Food Chemistry, 2007, 100 (2) :468-476.
|
[38] |
Asgar M A, Fazilah A, Huda N, et al.Nonmeat Protein Alternatives as Meat Extenders and Meat Analogs[J].Comprehensive Reviews in Food Science and Food Safety, 2010, 9 (5) :513-529.
|
[39] |
Soares S, Amaral J S, Oliveira M B, et al.Quantitative detection of soybean in meat products by a Taq Man real-time PCR assay[J].Meat Science, 2014, 98 (1) :41-46.
|
[40] |
Farrokhi R, Jafari J R.Identification of pork genome in commercial meat extracts for Halal authentication by SYBR green I real-time PCR[J].International Journal of Food Science&Technology, 2011, 46 (5) :951-955.
|
[41] |
Amaral J S, Santos G, Oliveira M B P P, et al.Quantitative detection of pork meat by Eva Green real-time PCR to assess the authenticity of processed meat products[J].Food Control, 2016, 72:53-61.
|
[42] |
Meira L, Costa J, Villa C, et al.Eva Green real-time PCR to determine horse meat adulteration in processed foods[J].LWTFood Science and Technology, 2016, 75:408-416.
|
[43] |
Soares S, Amaral J S, Mafra I, et al.Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay[J].Meat Science, 2010, 85 (3) :531-536.
|
[44] |
Soares S, Amaral J S, Oliveira M B, et al.A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products[J].Meat Science, 2013, 94 (1) :115-120.
|
[45] |
李莉, 蒋作明.PCR技术在食品沙门氏菌检测中的应用[J].食品科技, 2002 (4) :60-62.
|
[46] |
高正琴, 岳秉飞, 关伟鸿.CAR菌Taq Man MGB探针法实时荧光定量PCR快速检测方法的建立与应用[J].中国比较医学杂志, 2013, 23 (8) :9-13.
|
[47] |
Chin W H, Sun Y, Hgberg J, et al.Direct PCR-A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples.[J].Molecular&Cellular Probes, 2017, 32:24-32.
|
[48] |
Alves J, Hirooka E Y.Development of a multiplex real-time PCR assay with an internal amplification control for the detection of Campylobacter spp.and Salmonella spp.in chicken meat[J].LWT-Food Science and Technology, 2016, 72:175-181.
|
[49] |
邵美丽, 许岩, 刘思国, 等.Taq Man探针实时定量PCR检测肉中金黄色葡萄球菌的研究[J].食品工业, 2013, 34 (3) :109-112.
|
[50] |
Sakalar E, Mol S.Determination of irradiation dose and distinguishing between irradiated and non irradiated fish meat by real-time PCR[J].Food Chemistry, 2015, 182:150-155.
|
[51] |
Navarro E, Serrano-Heras G, Casta1o M J, et al.Real-time PCR detection chemistry[J].Clinica Chimica Acta, 2015, 439 (439) :231-250.
|
[52] |
Lockley A K, Bardsley R G.DNA-based methods for food authentication[J].Trends in Food Science&Technology, 2000, 11 (2) :67-77.
|
[53] |
Kelly F, Bhave M.Application of a DNA-based test to detect adulteration of bread wheat in pasta[J].Journal of Food Quality, 2007, 30 (30) :237-252.
|
[54] |
Koppel R, Zimmerli F, Breitenmoser A.Heptaplex real-time PCR for the identification and quantification of DNA from beef, pork, chicken, turkey, horse meat, sheep (mutton) and goat[J].European Food Research and Technology, 2009, 230 (1) :125-133.
|
[55] |
Hird H, Chisholm J, Sanchez A, et al.Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction[J].Food Additives&Contaminants, 2006, 23 (7) :645-650.
|
[56] |
Primrose S, Woolfe M, Rollinson S.Food forensics:methods for determining the authenticity of foodstuffs[J].Trends in Food Science&Technology, 2010, 21 (12) :582-590.
|