Preparation of high-purity Nisin and the optimization of industrial scale production conditions

In this study, a new method of preparation high-purity Nisin preparation was researched.The method adopted static adsorption and dynamic desorption of Nisin concentrated solution at a weak acidic ion exchange resin D113.The subsequent production process included salting out, suction filtration, drying and crushing to obtain high-purity Nisin. The results showed that the optimum condition was p H6, adsorption time 90 min, CDesorption agent= 1 mol/L, VHCl/VEt OHl= 1.25, desorption rate1 BV/h, CNa Cl= 23% in salting out.The potency of Nisin attained through this procedure was 34000 IU/mg, total yield reached67.45%.The method has bright prospect in industrial application.