Development of quantitative detection techniques of genetically modified organisms

DU Zhi-xin; JIAO Yue; ZHANG Liang-liang; ZHU Peng-yu; FU Wei

doi:10.13386/j.issn1002-0306.2017.10.065

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Science and Technology of Food Industry > 2017 > (10): 379-384. > DOI: 10.13386/j.issn1002-0306.2017.10.065

DU Zhi-xin, JIAO Yue, ZHANG Liang-liang, ZHU Peng-yu, FU Wei. Development of quantitative detection techniques of genetically modified organisms[J]. Science and Technology of Food Industry, 2017, (10): 379-384. DOI: 10.13386/j.issn1002-0306.2017.10.065

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Development of quantitative detection techniques of genetically modified organisms

The Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine
Guangxi Entry-Exit Inspection and Quarantine Bureau
Science and Technology Development Center, MOA
Hainan Entry-Exit Inspection and Quarantine Bureau

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Received Date: October 17, 2016

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Abstract

The concern of public has been more and more focus on the safety of the Genetically Modified Organisms ( GMO) as the rapid development of transgenic techniques and uncertain effect to human beings and environment. Different countries and organizations have declared kinds of safety and labeling laws to achieve the proper regulation to the commercialization of the GM products.In this manner, the achievement of stable and accurate quantitation of GMO content within different kinds of commercialized product has become the key point of the development of GMO detection techniques. In this paper, the application and development of different quantitative detection methods were summarized, such as competitive PCR, real-time PCR, digital PCR and etc. Meanwhile, the advantages and drawbacks of different detection methods were discussed and the future of the quantitative detection methods in the area of GMO content quantitation were predicted.
- transgenic,
- quantitative detection,
- PCR,
- digital PCR

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