Study on recycling technology and antioxidant activities of total flavones of sweet tea

The recycling technology of flavonoids was explored meanwhile unchanging original production of rubusoside. The removal capacity of the purified and unpurified flavonoids of sweet tea on·OH, DPPH·were studied by vitamin C as reference.Experiments were conducted in the laboratory according to production process in the factories and the contents of the total flavone in different parts were determined by using UV method.The results showed that the elution rate of flavonoids from the polyamide resin was up to 91.33% by 75% ethanol as elution liquid and content of flavonoids was up to 36.3%.75% ethanol desorption solution was purified by FL-3 resin further, the content of flavonoids in the final product was up to 62.70%, the yield was 1%.There were rutin and quercetin in the final flavonoids product by UV and HPLC analysis.Flavonoids compounds in sweet tea had good oxidation resistance.·OH scavenging ability were in order: unpurified flavonoids > purified flavonoids >vitamin C.DPPH· scavenging ability of unpurified flavonoids, purified flavonoids and vitamin C were almost the same when the concentration was more than 0.08 mg/m L, ability of DPPH· scavenging were in order: unpurified flavonoids > purified flavonoids > vitamin C when concentration is less than 0.08 mg/m L.