Establishment of mice model for intestinal dysbacteria induced by cefixime dispersible tablets

Objective: To produce the mouse models of intestinal dysbacteria which induced by cefixime dispersible tablets.Methods: female SPF grade BALB/c 36, weight 18~22 g, intestinal dysbiosis mice treated with cefixime dispersible tablets, low dose ( 187.75 mg/ ( kg· d) ) , medium dose ( 375.5 mg/ ( kg·d) ) and high dose ( 563.25 mg/ ( kg·d) ) . Control mice received sterile deionized water by oral gavage. On the 8th day, the mice were euthanized by cervical dislocation. The caecum contents were collected in a sterile 2 m L centrifuge tube. Compare the shift in the intestinal microbial by vital cell counting and polymerase chain reaction-denaturing gradient gel electrophoresis ( PCR-DGGE) analysis. Result: The Enterococcus and E. coli were promoted and Lactobacillus was suppressed. The reduction of diversity and abundance was shown in middle and higher doses group.Conclusion: The model of intestinal dysbacteria builded successfully.