Research of the surface plasmon resonance immunosensor for rapid detection of microcystin

As the existing pesticide detection methods were complicated, would pollute the environment by marking, with precious instrument, and not conducive to on-site rapid detection, the self-developed surface plasmon resonance biological chip detector was used in the detection of microcystin ( MC-LR) , a method for rapid detection of microcystin by inhibiting type SPR biological chip was proposed.The competitive inhibition immunoassay was preceded in MC-LR with concentrations of 3.5, 2.5, 1.5, 1, 0 μg/L.The experimental results showed that the detection limit could be 1 μg/L which could meet the needs of the World Health Organization ( WHO) for detecting the minimum content of MC-LR in drinking water and surface water environmental quality standards in China.The detection of 1 sample would take about 8 min by this method, compared with the traditional methods such as high performance liquid chromatography ( HPLC) and enzyme linked immunosorbent assay ( ELISA) , the rapid and quantitative was the biggest advantage of this method.The established device and method could be used for food quality monitoring and on-site real-time detection.