LIN Kuan, XU Cong-yue, LIANG Zheng, RU Qin, LI Chao-ying. Extraction, purification and antioxidant activity of polysaccharides from Opuntia Milpa Alta in vitro[J]. Science and Technology of Food Industry, 2017, (05): 140-144. DOI: 10.13386/j.issn1002-0306.2017.05.018
Citation: LIN Kuan, XU Cong-yue, LIANG Zheng, RU Qin, LI Chao-ying. Extraction, purification and antioxidant activity of polysaccharides from Opuntia Milpa Alta in vitro[J]. Science and Technology of Food Industry, 2017, (05): 140-144. DOI: 10.13386/j.issn1002-0306.2017.05.018

Extraction, purification and antioxidant activity of polysaccharides from Opuntia Milpa Alta in vitro

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  • Received Date: October 27, 2016
  • Objective: To investigate the purification of polysaccharides from Opuntia Milpa Alta and the antioxidant activity in vitro. Methods: Crude polysaccharides from Opuntia Milpa Alta ( MAP) was extracted by water extraction and alcohol sedimentation.Two polysaccharides named MAP1 and MAP2 were achieved after MAP was purified through DEAE-cellulose-52, and another polysaccharide named MAP3 was achieved through DEAE-Sepharose-CL-6B. The relative molecular mass of polysaccharides was analyzed by gel permeation chromatography ( GPC) and the antioxidant activity was investigated in vitro.Results: The relative molecular mass ( Mn) of MAP1 was 476.9 ku, MAP2 contained three relative molecular mass ( Mn) 5449, 9724, 529 ku, MAP3 contained 13270, 15.87, 474 ku. The results of antioxidant activity in vitro showed that, MAP had the highest total antioxidant capacity, the activity of MAP2 and MAP3 were higher than MAP in scavenging hydroxyl radical, superoxide anion and DPPH radical, and MAP2 had a higher clearance rate than MAP3 in the whole antioxidant indicators.Conclusion: The results indicated that MAP2 had a better antioxidant activity, while antioxidant activity of polysaccharide in vitro was associated with relative molecular mass.
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  • [1]
    沈文, 单承英, 张卫明.米邦塔仙人掌的研究进展与开发应用[J].中国野生资源, 2014, 33 (1) :4-8.
    [2]
    Gao J, Han Y L, Jin Z Y, et al.Protective effect of polysaccharides from Opuntia dillenii Haw fruits on streptozotocin-induced diabetic rats[J].Carbohydr Polym, 2015, 124:25-34.
    [3]
    Kang J W, Shin J K, Koh E J, et al.Opuntia ficus-indica seed attenuates hepatic steatosis and promotes M2 macrophage polarization in high-fat diet-fed mice[J].Nutrition Research, 2016, 36:369-379.
    [4]
    Antunes-Ricardo M, Gutiérrez-Uribe J A, López-Pacheco F, et al.In vivo anti-inflammatory effects of isorhamnetin glycosides isolated from Opuntia ficus-indica (L.) Mill cladodes[J].Industrial Crops and Products, 2015, 76:803-808.
    [5]
    罗川, 张万年.米邦塔仙人掌化学成分研究[J].Chinese Traditional Aand Herbal Drugs, 2011, 42 (3) :437-439.
    [6]
    缪月秋, 顾龚平, 吴国荣.植物多糖水解及其产物的研究进展[J].中国野生植物资源, 2005, 24 (2) :4-7.
    [7]
    李尔春, 丁红军, 金晓辉.天然植物多糖的结构及活性研究进展[J].食品与药品, 2007, 8 (4) :51-54.
    [8]
    Lefsih K, Delattre C, Pierre G, Michaud P, et al.Extraction, characterization and gelling behavior enhancement of pectins from the cladodes of Opuntia ficus indica[J].International Journal of Biological Macromolecules, 2016, 82:645-652.
    [9]
    Tian L, Zhao Y, Guo C, et al.A comparative study on the antioxidant activities of an acidic polysaccharide and various solvent extracts derived from herbal Houttuynia cordata[J].Carbohydrate Polymers, 2011, 83:537-544.
    [10]
    Schepetkin I A, Xie G, Kirpotina L N, et al.Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha[J].International Immunopharmacology, 2008, 8:1455-1466.
    [11]
    孙璇, 王仲孚, 黄琳娟.米邦塔仙人掌胶多糖化学组成的检测[J].西北大学学报, 2010, 40 (2) :273-276.
    [12]
    邱秀琴.自由基与抗氧化剂对人体健康的再评价[J].实用医技杂志, 2007, 14 (32) :4498-4500.
    [13]
    Berraaouan A, Abderrahim Z, Hassane M, et al.Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L.MILL.) against alloxan-induced diabetes in mice[J].Asian Pacific Journal Of Tropical Medicine, 2015, 8:532-537.
    [14]
    Yang N, Zhao M, Zhu B, et al.Anti-diabetic effects of polysaccharides from Opuntia monacantha cladode in normal and streptozotocin-induced diabetic rats[J].Innovative Food Science&Emerging Technologies, 2008, 9:570-574.
    [15]
    Cai W, Gu X, Tang J.Extraction, purification and characterization of the polysaccharides from Opuntia milpa alta[J].Carbohydrate Polymers, 2008, 71:403-410.
    [16]
    Sepúlveda E, Sáenz C, Aliaga E, et al.Extraction and characterization of mucilage in Opuntia spp[J].Journal of Arid Environments, 2007, 68:534-545.
    [17]
    Chen Y, Zhao B, Huang X, et al.Purification and neuroprotective effects of polysaccharides from Opuntia Milpa Alta in cultured cortical neurons[J].International Journal of Biological Macromolecules, 2011, 49:681-687.
    [18]
    蔡为荣, 顾小红, 汤坚.仙人掌多糖提取纯化及其抗凝血活性研究[J].食品科学, 2010, 31 (6) :131-136.
    [19]
    董群, 郑丽伊, 方积年.改良的苯酚硫酸法测定多糖和寡糖含量的研究[J].中国药学杂志, 1996, 31 (9) :550-553.
    [20]
    王文平, 郭祀远, 李琳, 等.野木瓜多糖中糖醛酸含量测定[J].工艺技术, 2007, 32 (10) :85-86.
    [21]
    何钢, 刘嵬, 李会萍, 等.银杏叶多糖分离纯化结构鉴定及抗氧化活性研究[J].食品工业科技, 2015, 36 (22) :81-86.
    [22]
    Zhang D Y, Luo M, Wang W, et al.Variation of active constituents and antioxidant activity in pyrola[P.incarnata Fisch.]from different sites in Northeast China[J].Food Chemistry, 2013, 141:2213-2219.
    [23]
    郭晓强, 何钢, 倩姚, 等.普通念珠藻中多糖的分离纯化研究[J].现代药物与临床, 2013, 28 (3) :328-330.
    [24]
    何念武, 杨兴斌, 田灵敏, 等.黄瓜多糖的体外抗氧化活性[J].食品科学, 2011, 32 (19) :70-74.
    [25]
    Zhao L, Dong Y, Chen G, et al.Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum[J].Carbohydrate Polymers, 2010, 80:783-789.
    [26]
    万建华, 顾洪雁.马铃薯渣果胶多糖分离纯化及结构初探[J].安徽农业科学, 2008, 36 (19) :7970-7973.

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