Optimization of microwave assisted enzyme method extraction technology of polysaccharide from shaddock peel by response surface method

Microwave assisted enzymaticd was used to extract polysaccharide from shaddock peel.On the basis of single factor test,the cellulase- to- pectinase ratio,microwave time and microwave power were selected as independent variables to shaddock peel polysaccharide extraction rate response by using Box- Behnken experimental and response equation.The results showed that the optimal extraction conditions were the cellulaseto- pectinase ratio 1.3 ∶ 1,reaction temperature 50 ℃,enzymolysis time 30 min,p H3,solid- solvent ratio 1 ∶ 40,microwave power 720 W,microwave time 2.5 min.Shaddock peel polysaccharides extracted utilization rate under these conditions was 22.8%.