Study the extraction and antioxidant activity of ginger glycoprotein

On the base of single factor experiment,the optimum extraction technology of ginger glycoprotein was performed by the quadratic regression orthogonal combination design,and the antioxidant activity of ginger glycoprotein was examined.The optimized condition of ginger glycoprotein was as follows: extraction temperature90 ℃,liquid- solid ratio 35.5∶ 1 and extraction p H value 7.5.At the optimum conditions,the yield of protein reached2.437 mg / g,and the yield of saccharide reached 117.126 mg / g. The typical characteristic absorption peaks of polysaccharide and protein were analyzed by the IR spectrum.The results of antioxidant tests and lectin microarray experiment demonstrated that the ginger glycoprotein has important physiological activities.