Optimization of cashew nuts protein extraction using response surface methodology

The cashew nuts produced abundantly by Hainan were used to be the research object,the alkaline extraction and acid precipitation technology of extraction process was optimized with solid- to- liquid( S / L) ratio,solvent p H,extraction temperature and time by single- factor and response surface methodology designs. The results showed that the best experimental conditions were found to be extraction at 40 ℃ for 1.5 h using an extraction solvent at p H11 with S / L ratio of 1 ∶ 50( g / m L).Under the optimized conditions,the yield of protein was87.1% ± 1.05%.The cashew nuts protein extraction regression model was highly significant( R~2= 0.9406),with good fitting and could be used to predict protein extraction rate.