Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA

YANG Xiao-ming; LIU Pan-jun; LI Yue-ying; FANG Yang-yang; SHI Ya-xiang

doi:10.13386/j.issn1002-0306.2016.20.013

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Science and Technology of Food Industry > 2016 > (20): 110-114. > DOI: 10.13386/j.issn1002-0306.2016.20.013

YANG Xiao-ming, LIU Pan-jun, LI Yue-ying, FANG Yang-yang, SHI Ya-xiang. Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA[J]. Science and Technology of Food Industry, 2016, (20): 110-114. DOI: 10.13386/j.issn1002-0306.2016.20.013

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Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA

School of Food and Biological Engineering,Jiangsu University
School of Medicine,Jiangsu University
School of Chemistry and Chemical Engineering,Jiangsu University
Traditional Chinese Hospital

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Received Date: April 13, 2016

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Abstract

Objective: To study the interaction between Ginkgol C15 ∶ 1 and Herring Sperm DNA. Methods: The interaction between Ginkgol C15 ∶ 1 and Herring Sperm DNA was investigated by fluorescence spectroscopy,viscosity measurements and thermal dissolution assay in the Tris- HCl buffer( p H7.4) at 293 K and 310 K,Acridine Orange( AO) as the fluorescent probe.Results: The fluorescence quenching of Ginkgol C15 ∶ 1 by AO- DNA was a static quenching procedure. The main binding force was hydrogen bond force on the basis of thermodynamic parameters,which indicated the interaction between Ginkgol C15 ∶ 1 and AO- DNA was intercalation. Conclusions:The results of viscosity measurements and the thermal denaturation experiments further demonstrated that intercalative binding was the main mode between Ginkgol C15∶ 1 and AO- DNA.
- Ginkgol C15∶ 1,
- Herring Sperm DNA,
- fluorescence spectrum

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Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA

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